| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,691,828 | T→G | 19.5% | intergenic (+53/‑118) | PP_1487 → / → PP_1488 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,691,828 | 0 | T | G | 19.5% | 52.6 / 9.1 | 30 | intergenic (+53/‑118) | PP_1487/PP_1488 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base T (10/14); new base G (4/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
TACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGA > NC_002947/1691689‑1691958 | tACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTcc < 2:376033/150‑1 (MQ=255) ccTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGt > 2:323077/1‑150 (MQ=255) tGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGtct < 2:481117/150‑1 (MQ=255) gACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGCCCGAGCGCCGCCCGTGCGGCGCTTCGTCTca < 2:180996/150‑1 (MQ=255) aCGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCACGGGCGGCGCTCCGTCTcac > 1:31553/1‑150 (MQ=255) gCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGgc > 1:411716/1‑150 (MQ=255) cTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGgcg < 2:386117/150‑1 (MQ=255) cTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGgcg > 1:73606/1‑150 (MQ=255) gCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAAtt > 1:573365/1‑150 (MQ=255) aGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCt < 2:42195/150‑1 (MQ=255) aCCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGAGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcac > 1:592667/1‑150 (MQ=255) ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACAt > 2:592381/1‑150 (MQ=255) cAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAg < 2:73606/150‑1 (MQ=255) aGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGa > 2:68145/1‑150 (MQ=255) gAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGAcc > 1:572380/1‑150 (MQ=255) tGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcac > 2:41740/1‑129 (MQ=255) tGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcac < 1:41740/129‑1 (MQ=255) tAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCta > 1:422834/1‑126 (MQ=255) tAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCta < 2:422834/126‑1 (MQ=255) aGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCCCATCAATACCTTATGATCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGt < 2:426533/150‑1 (MQ=255) gTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGtt < 1:592381/150‑1 (MQ=255) gCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTTCTGa < 2:411716/150‑1 (MQ=255) cTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGaa < 1:68145/150‑1 (MQ=255) tGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAAtgtg < 2:572380/150‑1 (MQ=255) gAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTga < 2:573365/150‑1 (MQ=255) cTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACt > 1:329428/1‑150 (MQ=38) gacggAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTgaa < 2:158615/135‑1 (MQ=38) gacggAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTgaa > 1:158615/5‑139 (MQ=38) ggAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGa > 2:120072/2‑144 (MQ=39) ggAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGa < 1:120072/143‑1 (MQ=39) | TACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGA > NC_002947/1691689‑1691958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |