Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,955,252	T→A	22.2%	intergenic (+39/‑154)	PP_1752 → / → PP_1753	hypothetical protein/DMT superfamily permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,955,252	0	T	A	22.2%	50.2 / 13.0	27	intergenic (+39/‑154)	PP_1752/PP_1753	hypothetical protein/DMT superfamily permease
Reads supporting (aligned to +/- strand):  ref base T (9/12);  new base A (4/2);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

TTGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATC  >  NC_002947/1955104‑1955396                                                                                                                                                     |                                                                                                                                                 ttGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGttt                                                                                                                                                 >  1:410570/1‑150 (MQ=255)    ccccTTGGACGCTGGTGGAGGCGAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGGACAATCAACCCGGCGCTTGAGGCCGCGTTTGCCAAGCCCAAGGAATAGGCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTAcc                                                                                                                                              <  2:104855/150‑1 (MQ=255)             cGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGATTGAGGCGGACTTTGGCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAgg                                                                                                                                     <  1:493769/150‑1 (MQ=255)                     gAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGcac                                                                                                                             <  1:224485/150‑1 (MQ=255)                           aaTGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCaa                                                                                                                       <  1:432333/150‑1 (MQ=255)                           aaTGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTGCTGACCCTTTCGCGGGTAAACCCGCGaagcggc                                                                                                                                   <  1:219815/138‑6 (MQ=37)                           aaTGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTGCTGACCCTTTCGCGGGTAAACCCGCGaagcggc                                                                                                                                   >  2:219815/1‑133 (MQ=37)                                    cgcTGGGCGCGGGTGAAGGTGGAGCAGGCAATGAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCAc                                                                                                              <  1:204/150‑1 (MQ=255)                                              gggTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGc                                                                                                    >  1:192017/1‑150 (MQ=255)                                                    aGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCaaa                                                                                              <  2:46535/150‑1 (MQ=255)                                                     ggtggtGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCaaaa                                                                                             <  1:263434/150‑1 (MQ=255)                                                                 aaTCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAGGAAATAGCCG‑AGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCtgatg                                                                                >  2:258161/1‑150 (MQ=255)                                                                 aaTCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGa                                                                                           >  2:438094/1‑140 (MQ=255)                                                                 aaTCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGa                                                                                           <  1:438094/140‑1 (MQ=255)                                                                                           ttGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCAcc                                                       <  2:410570/150‑1 (MQ=255)                                                                                              ccAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTATTTTCTCGGCACTCTCCACCGtc                                                    >  1:612107/1‑150 (MQ=255)                                                                                                        gAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGtcctcc                                          >  1:87246/1‑150 (MQ=255)                                                                                                           aTAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCATCTCGTATGTGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAAc                                       >  1:469336/1‑150 (MQ=255)                                                                                                                cGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAAcccc                                    <  2:25323/148‑1 (MQ=255)                                                                                                                cGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAAcccc                                    >  1:25323/1‑148 (MQ=255)                                                                                                                cGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGt                                  >  2:431724/1‑150 (MQ=255)                                                                                                                               cTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAAccc                   >  2:603072/1‑150 (MQ=255)                                                                                                                                         cctttCGCGGGTAAACCCGCGAAGGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACggg         >  1:490179/4‑150 (MQ=255)                                                                                                                                         cctttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGtct                                                   >  2:268327/4‑108 (MQ=39)                                                                                                                                         cctttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGtct                                                   <  1:268327/105‑1 (MQ=39)                                                                                                                                          gCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGc        <  2:482584/150‑1 (MQ=255)                                                                                                                                          ctttCGCGGGTAAACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGc        >  2:532417/3‑150 (MQ=255)                                                                                                                                                cgGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATc  <  1:431724/150‑1 (MQ=255)                                                                                                                                                    tttACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCAcc                                                       >  2:339525/1‑93 (MQ=255)                                                                                                                                                    tttACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCAcc                                                       <  1:339525/93‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTGCCCCTTGGACGCTGGTGGAGGCCAATGACAAGCGCTGGGCGCGGGTGAAGGTGGTGCGCACAATCAACCAGGCGCTTGAGGCGGCGTTTGCCAAGCACAAGAAATAGCCGAAGGTGAGGTTGCCTGTTCTGGCCGCTTCGCGGGTTTACCCGCGAAAGGGTCAGCACAGGCAACACCTCCACCCAGCCATGCCCCAAAAGAATGACCTGATGAATTCTTTTCTCGGCACTCTCCACCGTCTCTGTCCTCCAACCCCGTAGAATTCAACAACCCCCACCACGGGCTTGATC  >  NC_002947/1955104‑1955396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑