| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,092,458 | C→T | 16.7% | intergenic (+61/+30) | deaD → / ← ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,458 | 0 | C | T | 16.7% | 44.8 / 4.2 | 24 | intergenic (+61/+30) | deaD/ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (9/11); new base T (2/2); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
GACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCTCATCA > NC_002947/2092311‑2092602 | gACGGCCTCGAGAAGCTGCAGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GGCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCtt > 1:19150/1‑150 (MQ=255) gCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGTTCCTGGCGTAACGAAAAAACCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcgc < 2:249264/150‑1 (MQ=255) aaGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGc < 2:225002/150‑1 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaaggg > 1:396213/1‑146 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAaga < 2:39105/148‑1 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAaga > 1:39105/1‑148 (MQ=255) aaGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaagggc < 2:396213/150‑6 (MQ=255) ggACTCTCGCGTGGCTGGCAAGCAGCTGACGATGCAGGCG‑TACCGCGAGGATTGATCCTTGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGt < 2:299634/150‑1 (MQ=255) ggctggcAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCTTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGa < 1:187707/150‑1 (MQ=255) tggcAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaagg < 1:562710/125‑4 (MQ=38) tggcAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCTCTTCGCGGGCAAGCCCGCGaaagg > 2:562710/1‑122 (MQ=38) ggcAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTc < 1:521678/150‑1 (MQ=255) gcAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTcc > 1:176985/1‑150 (MQ=255) gCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTa < 2:425853/150‑1 (MQ=255) gAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTg > 1:313833/1‑150 (MQ=255) aTTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGc > 2:579350/1‑150 (MQ=255) tCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGc < 2:291540/87‑1 (MQ=255) tCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGc > 1:291540/1‑87 (MQ=255) cGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGaa < 1:579350/150‑1 (MQ=255) aaaaaaTCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCACTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCCGCGCGCCCATGGTAAACCCCTTATGCACCACGCCTAACTTGCTc > 2:76483/1‑150 (MQ=255) aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTcc > 1:209774/1‑150 (MQ=255) ccccGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGccc < 2:209774/150‑1 (MQ=255) ggTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCaaa < 1:51247/150‑1 (MQ=255) cGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCtcatca > 2:124399/1‑150 (MQ=255) | GACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGC‑GTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGCAAAGAACAGCGGCAGCAAATGCTCATCA > NC_002947/2092311‑2092602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |