| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,104,740 | A→G | 14.8% | F63L (TTC→CTC) | PP_1879 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,104,740 | 0 | A | G | 14.8% | 54.8 / 3.3 | 27 | F63L (TTC→CTC) | PP_1879 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/11); new base G (2/2); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
TTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGT > NC_002947/2104601‑2104877 | ttGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAAGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAGTTCCACCCGc < 2:150782/150‑1 (MQ=255) ccGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAgggggg < 1:9676/150‑1 (MQ=255) aaaatGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGtt > 2:195575/4‑150 (MQ=255) ttCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCAAACCAGTAGAAAAAAAAATATACCTCAACAATTTCCGTGAGTGAATTTGCCTACTGGGGGAAGTTCTTTCGCAGGGTTGAATAGGTTGCCCGGAACTCGACCGTGtt < 2:70372/150‑1 (MQ=255) gATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAGCTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTggg > 1:518075/1‑150 (MQ=255) aTAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGt > 2:60062/1‑150 (MQ=255) tGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCtt > 1:368236/1‑150 (MQ=255) aaGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGtt < 2:530718/139‑1 (MQ=255) aaGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGtt > 1:530718/1‑139 (MQ=255) aGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCaa < 1:60062/150‑1 (MQ=255) ttcttGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCg > 2:325908/1‑150 (MQ=255) cttGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAGCTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTc < 2:518075/150‑1 (MQ=255) aCCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACg > 1:597833/1‑150 (MQ=255) gTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTggggg < 2:440996/150‑1 (MQ=255) tAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAAATCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTgggggg > 2:494681/1‑150 (MQ=255) tCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTcc > 1:423512/1‑150 (MQ=255) aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTg > 2:614876/1‑62 (MQ=255) aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACgg < 1:496981/122‑1 (MQ=255) aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACgg > 2:496981/1‑122 (MQ=255) aTCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGCTg < 1:614876/62‑1 (MQ=255) tttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGa < 2:133624/126‑1 (MQ=255) tttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGa > 1:133624/1‑126 (MQ=255) tttCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCCATGGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAg < 1:195575/150‑1 (MQ=255) ccGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGaa < 2:50017/47‑1 (MQ=255) ccGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGaa > 1:50017/1‑47 (MQ=255) tGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGa < 2:597833/150‑1 (MQ=255) ccctgcGGGTGGAGTTCCACCCG‑TGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGt > 1:360697/6‑150 (MQ=255) | TTGTCGACCGGTACCAATATGAATAGCTCTTCAGGTTCGATAGGCTTGCTGAAGGTCTTCTTGTCGACCAGTACGTCATTCCAGTAGAGGAAAAGGTATTCCTCATCTATTTCCGGGTGCGAATTTGCCCACGGGTGGAACTCCACCCGCAGGGGGGAATAGGTTGCCCGGAACTCGACCGTGTTGGGTTTCCCCCCTTCAACATCGTCCACCACGCTTGGTACGGTTGGGGGGACAAGAGAGGCCGAGTCCAGTTCAGGGGTTGGAGTTCTGCGGT > NC_002947/2104601‑2104877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |