| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,254,045 | G→C | 16.0% | P107P (CCG→CCC) | leuB → | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,254,045 | 0 | G | C | 16.0% | 58.4 / 6.5 | 25 | P107P (CCG→CCC) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base C (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
CCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGTGCCCCGCGTGGCCAGCGCGAGCTGGAAGGTG > NC_002947/2253901‑2254184 | ccTGGAGCGTGCGCGCAACGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGAACGAGCGAGACATTCGCCCGGAGCGCCGCCTGCTGAAGATCTGCTCGCAACTG‑GGCCTGTTCTCCAAC‑CTGCGCCCGGCCAt > 2:354292/1‑150 (MQ=255) cTGGAGCGTGCGCGCAAGGTCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATc < 2:406370/150‑1 (MQ=255) aaGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACta > 1:93883/1‑149 (MQ=255) cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCCGccctt > 1:285464/1‑127 (MQ=255) cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCCGccctt > 1:285601/1‑127 (MQ=255) cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCCGccctt < 2:285601/130‑4 (MQ=255) cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCCGccctt < 2:285464/130‑4 (MQ=255) tgGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGa > 1:519036/1‑150 (MQ=255) gggCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGc < 2:214299/150‑1 (MQ=255) cTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCgg < 2:551531/150‑1 (MQ=255) gggcgggcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑CGCCTGTTCGCCAACGCCGC‑CCCGGCCAGCCGTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAAt < 1:537723/150‑1 (MQ=255) gcCGAAGTGGGACCCGATCGAGCTAAACATTCTCCCGGAGCGCGGCCTGCTGAAGATCCGCTCG‑AATTGCGTCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc < 1:289880/150‑1 (MQ=255) gcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc > 2:607588/1‑150 (MQ=255) gcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc > 1:535097/1‑150 (MQ=255) gagcgagACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCAtt > 1:128761/1‑150 (MQ=255) aTTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTAc < 2:139505/78‑1 (MQ=255) aTTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTAc > 1:139505/1‑78 (MQ=255) cgGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCggg < 1:427343/111‑1 (MQ=255) cgGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCggg > 2:427343/1‑111 (MQ=255) aaGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGt > 2:276271/1‑150 (MQ=255) aaGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGt > 2:239088/1‑150 (MQ=255) gCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGAc < 2:31363/120‑1 (MQ=255) gCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGAc > 1:31363/1‑120 (MQ=255) aCTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGTGCCCCGCGTGGCCa < 1:239088/150‑1 (MQ=255) aC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGTGCCCCGCGTGGCCAGCGCGAGCTGGAAGGTg < 2:535097/150‑1 (MQ=255) | CCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTG‑GGCCTGTTCGCCAAC‑CTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTCGGGCCTGGACATCCTCATTGTCCGTGAGCTGACCGGCGGCATCTACTTCGGTGCCCCGCGTGGCCAGCGCGAGCTGGAAGGTG > NC_002947/2253901‑2254184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |