Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,568,736 T→A 18.4% Q285H (CAA→CAT)  PP_2252 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,568,7360TA18.4% 88.6 / 15.1 38Q285H (CAA→CAT) PP_2252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/14);  new base A (2/5);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

GCTGCTCCACCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTC  >  NC_002947/2568588‑2568885
                                                                                                                                                    |                                                                                                                                                     
gctgctCCACCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGCCCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAgtt                                                                                                                                                      <  1:382485/150‑1 (MQ=255)
   gctCCACCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAgttgtt                                                                                                                                                   <  2:203162/150‑1 (MQ=255)
         ccGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCa                                                                                                                                             >  1:552282/1‑150 (MQ=255)
            gCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCt                                                                                                                                          >  2:74461/1‑150 (MQ=255)
              cgcgGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACGCGCAGTTGTTCGACCATCTGc                                                                                                                                        >  1:364255/1‑150 (MQ=255)
                cggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                      <  2:499128/150‑1 (MQ=255)
                cggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGACGGTCGAACAACTGCgg                                                                                                                                      <  2:453168/150‑1 (MQ=255)
                    gcAGTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGggtgg                                                                                                                                  <  2:100895/150‑1 (MQ=255)
                       cTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCGGGTGATcc                                                                                                                               <  1:439986/150‑2 (MQ=255)
                          gCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACgg                                                                                                                            <  1:206056/150‑1 (MQ=255)
                          gCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACgg                                                                                                                            >  2:170271/1‑150 (MQ=255)
                            cAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTc                                                                                                                          <  1:523640/150‑1 (MQ=255)
                                   cTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtc                                                                                                                   >  1:362144/1‑150 (MQ=255)
                                     gTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtcat                                                                                                                 <  2:280815/150‑1 (MQ=255)
                                        cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                      >  2:62514/1‑126 (MQ=255)
                                        cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                      <  1:62514/126‑1 (MQ=255)
                                                           tAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCa                                                                                           >  2:292057/1‑150 (MQ=255)
                                                              tAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATc                                                                                        <  2:362144/150‑1 (MQ=255)
                                                                    cccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAatc                                                                                  >  2:595232/1‑150 (MQ=255)
                                                                              ccccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTg                                                                        <  1:492916/150‑1 (MQ=255)
                                                                                ccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGcc                                                                      >  1:152173/1‑150 (MQ=255)
                                                                                  gCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                      <  1:260694/100‑1 (MQ=255)
                                                                                  gCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                      >  2:260694/1‑100 (MQ=255)
                                                                                       ggCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGc                                                               <  1:161425/150‑1 (MQ=255)
                                                                                         cGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCgg                                                                                                                                      <  2:144913/77‑1 (MQ=255)
                                                                                         cGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCgg                                                                                                                                      >  1:144913/1‑77 (MQ=255)
                                                                                                  aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCACTGCCACCTTGCCCACTGCCGAt                                                    >  1:493888/1‑150 (MQ=255)
                                                                                                     aaCTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGcc                                                 <  1:190237/150‑1 (MQ=255)
                                                                                                     aaCTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGcc                                                 <  1:190208/150‑1 (MQ=255)
                                                                                                            gCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCAc                                          >  2:402266/1‑150 (MQ=255)
                                                                                                                ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGc                                      >  2:36242/1‑150 (MQ=255)
                                                                                                                ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCggg                                                                                                                                     >  1:151555/1‑55 (MQ=17)
                                                                                                                ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCggg                                                                                                                                     <  2:151555/55‑1 (MQ=17)
                                                                                                                  ccTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGa                                    <  2:552282/150‑1 (MQ=255)
                                                                                                                      gTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                >  1:170462/1‑150 (MQ=255)
                                                                                                                        tCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcgc                              <  2:493888/150‑1 (MQ=255)
                                                                                                                            cGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTgg                          >  1:2617/1‑150 (MQ=255)
                                                                                                                             gCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTggg                         <  1:74461/150‑1 (MQ=255)
                                                                                                                                  gTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGTTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAg                    >  1:594945/1‑150 (MQ=255)
                                                                                                                                    tCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAggg                  >  1:190585/1‑150 (MQ=255)
                                                                                                                                                    ttgttCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTc  <  1:170271/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTGCTCCACCGGCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTC  >  NC_002947/2568588‑2568885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑