| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,769,912 | G→C | 14.4% | A89G (GCC→GGC) | PP_2422 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,769,912 | 0 | G | C | 14.4% | 84.6 / 5.1 | 35 | A89G (GCC→GGC) | PP_2422 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/16); new base C (3/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AGCCGCTCAGTCTGGGTGGCGCGGCGCCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAGGTGGTTGGTCTTGGCACCT > NC_002947/2769766‑2770058 | aGCCGCTCAGTCTGGGTGGCGCGGCGCCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCt > 2:592430/1‑150 (MQ=255) tGGGTGGCGCGGCGCCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCt > 1:233345/1‑150 (MQ=255) gcggcgcCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTg < 2:600605/150‑1 (MQ=255) gcggcgcCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTg < 1:592430/150‑1 (MQ=255) cgcgCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCtt < 2:371394/150‑1 (MQ=255) gcAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGa < 1:328094/150‑1 (MQ=255) aaGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACg < 2:433043/150‑1 (MQ=255) tttGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCa < 2:599673/150‑1 (MQ=255) ttGTGCACAGGCGTTGGCCGTGCCGAGCGCGTCCATTGCACGGCCGCAGTACCCCAGCGCGGCACCGGAGTTGATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCAc < 1:168009/150‑1 (MQ=255) tCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGAt > 2:293834/1‑150 (MQ=255) tCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGAGCGGAATACACAAGCGCGGCACCGGCGTTCATGGCCACGGCAACGCCGAGGCCCTCGGCAAGTTCCTCGCGGCTCGCCCCCGCTTTGACCGCCTGCTACGCACGCTCGGCgca > 2:98100/1‑148 (MQ=255) aaTCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGACTGTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGc > 2:391857/1‑150 (MQ=255) ccTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGc > 1:292650/1‑150 (MQ=255) ccACCGCGTCCATTGCACGGGCGGAGTACACTAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCgag > 1:308188/1‑148 (MQ=255) gTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGggtg > 2:10429/1‑145 (MQ=255) gTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGggtg < 1:10429/145‑1 (MQ=255) tCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCAGGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCCGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTcacg < 1:98100/150‑2 (MQ=255) tCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTcaca > 1:92963/1‑150 (MQ=255) tCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTcaca > 1:470802/1‑150 (MQ=255) tGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGACGAGGTCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCAc < 1:409554/150‑1 (MQ=255) gCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAg > 1:362438/1‑150 (MQ=255) gCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAg < 2:233345/150‑1 (MQ=255) cacCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAg < 2:292650/150‑1 (MQ=255) acCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGAAACTCGCCTATCTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCCGCGGGTGCTCACAGCCACGGCCAAGgagaaccgc > 1:108631/1‑145 (MQ=255) aCCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCtt > 2:482994/1‑150 (MQ=255) ccGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTg < 2:129032/150‑1 (MQ=255) ggccacggccacGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAggtg < 2:92963/150‑1 (MQ=255) ggccacggccacGCCGAGGGCCTAGCCAGGTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAggtg > 1:478971/1‑150 (MQ=255) ttgCCGAGGCCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTcacc < 2:375718/94‑2 (MQ=255) ttgCCGAGGCCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTcacc > 1:375718/3‑95 (MQ=255) ttgCCGAGGCCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTg > 2:127141/3‑129 (MQ=255) ttgCCGAGGCCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTg < 1:127141/127‑1 (MQ=255) gCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAGGTGGTTGGTCTTGGc < 1:482994/150‑1 (MQ=255) ggAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCGCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGGCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAGGTGGTTGGTCTTGGCAc < 2:108631/149‑1 (MQ=255) aGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAGGTGGTTGGTCTTGGCACCt < 2:308188/150‑1 (MQ=255) | AGCCGCTCAGTCTGGGTGGCGCGGCGCCGTCGCGCAAGGGCGGCGACGCCGTTTGTGCTCAATCGTTGGCCTTGCCCACCGCGTCCATTGCACGGGCGGAGTACACCAGCGCGGCACCGGCGTTCATGGCCACGGCCACGCCGAGGGCCTCGGCAATTTCCTCGCGGCTGGCCCCCGCTTTGACGGCCTGCTGCGAATGCACGGCGATGCAGCCGTCGCAGCGGGTGGTCACAGCCACGGCCAAGGAGATCAGCTCGCGGGTCTTGGCATCCAGGTGGTTGGTCTTGGCACCT > NC_002947/2769766‑2770058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |