| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,116,218 | G→A | 15.4% | G305S (GGC→AGC) | PP_2732 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,116,218 | 0 | G | A | 15.4% | 57.0 / 4.2 | 26 | G305S (GGC→AGC) | PP_2732 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base A (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCG > NC_002947/3116073‑3116359 | gCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTg < 2:386238/150‑1 (MQ=255)gCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTg < 2:309304/150‑1 (MQ=255) cGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGc < 2:430459/150‑1 (MQ=255) gcgcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTgg > 1:393898/1‑150 (MQ=255) gcgcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTgg > 1:115997/1‑150 (MQ=255) gcATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCa > 1:399481/1‑150 (MQ=255) gTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTAtt > 1:270816/1‑150 (MQ=255) gTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTAtt < 1:602419/150‑1 (MQ=255) cgcTAAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGTCAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAgc < 1:37786/150‑1 (MQ=255) tGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCg < 2:504465/150‑1 (MQ=255) gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggg < 2:393898/150‑1 (MQ=255) tcTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggcatgc > 1:46732/1‑138 (MQ=255) tcTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggcatgc < 2:46732/143‑6 (MQ=255) cgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCg < 2:399481/150‑1 (MQ=255) aCAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgcgc > 1:460221/1‑150 (MQ=255) aCAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgcgc > 1:23726/1‑150 (MQ=255) ggTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCgg < 1:253619/150‑1 (MQ=255) tGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGaa > 2:335649/1‑124 (MQ=255) tGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGaa < 1:335649/124‑1 (MQ=255) ttCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGgct > 1:438246/1‑149 (MQ=255) tcacGCCAGCCGATCAGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCaa > 1:144685/4‑150 (MQ=255) acGCCAGCCGATCAGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgc > 2:368740/2‑96 (MQ=255) acGCCAGCCGATCAGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgc < 1:368740/95‑1 (MQ=255) agccgATCAGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCg < 2:144685/146‑1 (MQ=255) aTCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCgg > 2:527878/1‑84 (MQ=255) aTCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCgg < 1:527878/84‑1 (MQ=255) | GCCGTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCG > NC_002947/3116073‑3116359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |