| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,183,926 | T→C | 15.7% | V124A (GTG→GCG) ‡ | PP_2794 → | short‑chain dehydrogenase/reductase family oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,183,926 | 0 | T | C | 15.7% | 70.9 / 4.7 | 31 | V124A (GTG→GCG) ‡ | PP_2794 | short‑chain dehydrogenase/reductase family oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (14/12); new base C (3/2); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGCCCACCTGACCCGCGCCATGG > NC_002947/3183780‑3184075 | ggACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGc > 2:533574/1‑150 (MQ=255) ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAggcgc < 2:532521/127‑4 (MQ=255) ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAggcgc > 1:532521/1‑124 (MQ=255) ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAgg < 1:405719/124‑1 (MQ=255) ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAgg > 2:405719/1‑124 (MQ=255) cGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCgg < 1:573865/150‑1 (MQ=255) cGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTCCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCgg < 1:539088/150‑1 (MQ=255) tGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTACGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGa > 2:415310/1‑150 (MQ=255) gTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg < 2:261270/150‑1 (MQ=255) ttCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAggggg > 1:295464/1‑150 (MQ=255) gTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGc > 1:409699/1‑150 (MQ=255) aacaaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTg > 2:417571/1‑150 (MQ=255) acaaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGa > 2:32931/1‑150 (MQ=255) caaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGAt < 1:32931/150‑1 (MQ=255) aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCAtggtggt > 1:521852/1‑125 (MQ=255) aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCAtggtggt < 2:521852/125‑1 (MQ=255) aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATc < 1:533574/150‑1 (MQ=255) tGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCaa < 2:295464/150‑1 (MQ=255) tGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTACGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCaa < 1:415310/150‑1 (MQ=255) gCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAAt < 2:409699/150‑1 (MQ=255) aCAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATcc > 2:169265/1‑150 (MQ=255) ccagccTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGCGGACCTGCAAGGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGc > 1:500562/1‑150 (MQ=255) cTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg > 2:537147/1‑113 (MQ=255) cTTTGCTAGCCTGCGATGATCAAACCTGGGACCAAGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg < 1:537147/113‑1 (MQ=255) cTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCGGGGCCGTGGCCCAGGAAAGCACCCGGCGGATGGTGGTGGCGTTGAATTTGCCGGGGCTTATCAATGTCACCTCGATCCTCGCCAGCCGtgtg < 1:169265/150‑1 (MQ=255) cACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTggcg > 2:83241/1‑150 (MQ=255) cACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTggcg < 1:343754/150‑1 (MQ=255) ccAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAggccggcc > 2:565727/1‑150 (MQ=255) aaCCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTg > 1:367747/1‑150 (MQ=255) tCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGccc > 1:224218/1‑150 (MQ=255) gcCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCAGCCAAGGCCGGCCTGGCCCACCTAAcc > 1:282067/1‑150 (MQ=255) tGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGCCCACCTGACCCGCGCCATgg > 2:525107/1‑150 (MQ=255) | GGACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGCCCACCTGACCCGCGCCATGG > NC_002947/3183780‑3184075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |