Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,307,089 T→C 17.2% K185E (AAG→GAG)  csiR ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,307,0890TC17.2% 60.8 / 6.9 29K185E (AAG→GAG) csiRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (15/9);  new base C (3/2);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

GCGGGCACCGATGCCTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACGCCTTGTGCCGC  >  NC_002947/3306949‑3307237
                                                                                                                                            |                                                                                                                                                    
gCGGGCACCGATGCCTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTCAagcgccag                                                                                                                                             <  1:156631/150‑1 (MQ=255)
 cGGGCACCGATGCCTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagcgccagc                                                                                                                                            <  1:88268/150‑1 (MQ=255)
              cTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagc                                                                                                                                                  >  2:330345/1‑131 (MQ=255)
              cTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagc                                                                                                                                                  <  1:330345/131‑1 (MQ=255)
                        cATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCACTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCTTCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTg                                                                                                                     >  1:442445/1‑150 (MQ=255)
                         aTGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGc                                                                                                                    >  1:72225/1‑150 (MQ=255)
                              cTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAg                                                                                                               <  2:430734/150‑1 (MQ=255)
                                        aTCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagcgccagcgc                                                                                                                                          >  1:165565/1‑113 (MQ=255)
                                        aTCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGagcgccagcgc                                                                                                                                          <  2:165565/113‑1 (MQ=255)
                                            gTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGt                                                                                                 >  2:13308/1‑150 (MQ=255)
                                                       atcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTCAagcgccaggagc                                                                                                                                         <  2:423312/99‑5 (MQ=255)
                                                       atcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTCAagcgccaggagc                                                                                                                                         >  1:423312/1‑95 (MQ=255)
                                                        tcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCt                                                                                     >  1:13909/1‑150 (MQ=255)
                                                        tcaGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCt                                                                                     >  1:48277/1‑150 (MQ=255)
                                                            gTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTc                                                                                 >  2:419832/1‑150 (MQ=255)
                                                                                tGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCt                                                             >  1:350617/1‑150 (MQ=255)
                                                                                      ttttGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGc                                                                                       >  2:14452/1‑118 (MQ=255)
                                                                                      ttttGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGc                                                                                       <  1:14452/118‑1 (MQ=255)
                                                                                                               cTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAg                                                                                               <  1:534431/85‑1 (MQ=255)
                                                                                                               cTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAg                                                                                               >  2:534431/1‑85 (MQ=255)
                                                                                                                tCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCa                             >  2:348287/1‑150 (MQ=255)
                                                                                                                    ccAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATgg                         <  2:218051/150‑1 (MQ=255)
                                                                                                                    ccAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATgg                         >  1:218051/1‑150 (MQ=255)
                                                                                                                      aGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCa                       <  1:13308/150‑1 (MQ=255)
                                                                                                                       gTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTAGGAAAACAACGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCGTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAg                      >  2:70613/1‑150 (MQ=255)
                                                                                                                              aTGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGATGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGaa               <  2:357358/150‑1 (MQ=255)
                                                                                                                                gCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACg             >  1:509487/1‑150 (MQ=255)
                                                                                                                                    cTGGCGCTCAAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACGCCtt         >  2:248476/1‑150 (MQ=255)
                                                                                                                                    cTGGCGCTCAAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACGCCtt         >  1:312115/1‑150 (MQ=255)
                                                                                                                                           ttGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACGCCTTGTGCCGc  <  1:28711/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GCGGGCACCGATGCCTTCGGCGTGCATGATCTGCGCAATGATCGGTACCGGTGTCATCAGGTGCGAGCGCATCATGGCGCTGGCGGTTTTGGCATCCCGGGCGAGGATCACCTCGACCAGTGCCGCATGCTCCTGGCGCTTGAGCGCCAGCGCCTCTTCGGAAAACACCGTCTGCGTCAGCCACAGGTGGCGGTAGCGCTCGGCCTGGTCGAACAGGTAGGTGCGTGCCTGCAGCAGGTGCTTGGAGCCGCAGCCCGAGGCAATGGCAGTATGGAACGCCTTGTGCCGC  >  NC_002947/3306949‑3307237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑