| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,554,503 | G→C | 13.8% | E26Q (GAG→CAG) | PP_3139 → | group 1 family glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,554,503 | 0 | G | C | 13.8% | 72.7 / 6.2 | 29 | E26Q (GAG→CAG) | PP_3139 | group 1 family glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (16/9); new base C (2/2); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
CCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGA > NC_002947/3554355‑3554638 | cccGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGa > 2:524393/1‑150 (MQ=255) cGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTAGCACCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAgc < 1:315617/150‑1 (MQ=255) aCAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCCTGAATCAGTACCCGAAAGTCAGCTACAGTTTCATCCGTCGCGAAATACTGGCGCTGGTGCGCCAAggg > 2:116951/1‑150 (MQ=255) gtttGCCAAGGAAGGTTGGTCATCAAGTGTGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGGGCTGGAGCGCCAAGGGGTTGAGGTGCAACGt < 1:116951/148‑1 (MQ=255) ttGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGAGATGAGGTGCAACGtat > 2:34278/1‑150 (MQ=255) tcatcaAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGtgggtggg > 2:17285/1‑150 (MQ=255) aaGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGcc < 1:17285/150‑1 (MQ=255) aTCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGtt > 2:575234/1‑102 (MQ=255) aTCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGtt < 1:575234/102‑1 (MQ=255) tATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGc > 2:4678/1‑150 (MQ=255) ttACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCCAGCGAACgggg > 2:321767/1‑150 (MQ=255) tACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGc > 1:584448/1‑150 (MQ=255) tACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGt > 2:608671/1‑104 (MQ=255) tACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGt < 1:608671/104‑1 (MQ=255) ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAg < 2:598309/105‑1 (MQ=255) ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAg > 1:598309/1‑105 (MQ=255) ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAg > 1:597920/1‑105 (MQ=255) ccGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAg < 2:597920/105‑1 (MQ=255) gAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACgggg < 2:304557/129‑1 (MQ=255) gAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACgggg > 1:304557/1‑129 (MQ=255) cGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGttgttg > 1:512868/1‑150 (MQ=255) gAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAgg > 1:417713/1‑125 (MQ=255) gAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAgg < 2:417713/125‑1 (MQ=255) gAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCg > 1:143962/1‑150 (MQ=255) aaaTACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGa > 2:388843/1‑150 (MQ=255) cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGTGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTggg > 1:611105/3‑124 (MQ=255) cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGTGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTggg < 2:611105/122‑1 (MQ=255) cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg < 2:361509/77‑1 (MQ=255) cttGGCGCTCCAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAg > 1:361509/3‑79 (MQ=255) | CCCGTAGTCGACAGCCTGAAGTGAAGGTTGCCAGGGAAGGTTGGTCATCAAGCGGGTTTGGATCTGGATTCGTATGCGCATTGCTTACTTCATCAATCAGTACCCGAAAGTCAGCCACAGTTTCATCCGTCGCGAAATACTGGCGCTGGAGCGCCAAGGGGTTGAGGTGCAACGTATAGCCCTGCGTGGGTGGGACGCCGAAGTGCAGGATGCCGAGGACGCAAGCGAACGGGGCAAGACCCGCTACGTATTGCAAGGTGGGCTCAAGGGGTTGTTGGCACCGA > NC_002947/3554355‑3554638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |