| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,962,544 | T→A | 15.4% | L233Q (CTG→CAG) | PP_3492 → | acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,962,544 | 0 | T | A | 15.4% | 62.8 / 5.2 | 26 | L233Q (CTG→CAG) | PP_3492 | acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (9/13); new base A (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GCGTTCCTGGTGCCCACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACAC > NC_002947/3962396‑3962683 | gCGTTCCTGGTGCCCACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTg < 1:199243/150‑1 (MQ=255) tGGTGCCCACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAATGGCCAGGCCCTTg < 2:407090/150‑1 (MQ=255) gCCCACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCt < 2:480218/150‑1 (MQ=255) cACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTc > 2:109098/1‑150 (MQ=255) ccTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAATGGCCAGGCCCTTGccac < 2:508465/137‑3 (MQ=255) ccTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAATGGCCAGGCCCTTGccac > 1:508465/1‑135 (MQ=255) ccTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGaa < 1:52929/150‑1 (MQ=255) ggTTCAAGGTTGACCGCAGCGAAAACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAggcg < 1:460841/150‑1 (MQ=255) tCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGcc > 1:88868/1‑150 (MQ=255) ccGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTAt > 2:554819/1‑144 (MQ=255) ccGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTAt < 1:554819/144‑1 (MQ=255) cGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCAtt < 2:168124/150‑1 (MQ=255) gAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGGGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTgccgcc > 1:43053/1‑150 (MQ=255) tGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTgg < 1:109098/150‑1 (MQ=255) cATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCAt > 1:46248/1‑150 (MQ=255) aTTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATc < 2:535805/150‑1 (MQ=255) aTACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCg < 2:30744/138‑1 (MQ=255) aTACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCg > 1:30744/1‑138 (MQ=255) cTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTgcc < 2:398063/115‑1 (MQ=255) cTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTgcc > 1:398063/1‑115 (MQ=255) gCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGAcc > 1:484650/1‑150 (MQ=255) tGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACa > 1:488003/1‑150 (MQ=255) gccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGttt < 2:488003/150‑1 (MQ=255) ccTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGcc < 1:229825/150‑1 (MQ=255) tCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa < 2:484650/150‑1 (MQ=255) ggCAATGGCCAGGCCCTTGCCCTGTCCGACGTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAacac > 1:479111/1‑150 (MQ=255) | GCGTTCCTGGTGCCCACCGACAACCCTGGGTTCAAGGTTGACCGCAGCGAACACAAGATGGGCATTCGCGCCTCCGATACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAAGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACAC > NC_002947/3962396‑3962683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |