| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,042,365 | C→A | 20.1% | L137I (CTC→ATC) ‡ | PP_3563 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,042,365 | 0 | C | A | 20.1% | 36.4 / 5.1 | 20 | L137I (CTC→ATC) ‡ | PP_3563 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/11); new base A (2/2); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TCAGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGT > NC_002947/4042219‑4042510 | tCAGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCa < 2:548197/150‑1 (MQ=255) ccTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGA‑CTGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCGCGGCCGCTGAGCAGCGgc < 1:526939/150‑1 (MQ=255) acgcGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGc < 1:298382/150‑1 (MQ=255) gccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAg < 2:450998/150‑1 (MQ=255) ccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAgg < 1:523199/150‑1 (MQ=255) gcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGaa < 1:452768/150‑1 (MQ=255) catgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAg < 2:536335/150‑1 (MQ=255) aGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGGCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCagg > 2:112077/1‑150 (MQ=255) ttCTGCCAGGTGGTGCTCGACCCGCAATTCGTAGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAc > 1:63359/1‑150 (MQ=255) gtcgATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGc > 2:984/1‑89 (MQ=255) gtcgATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGc < 1:984/89‑1 (MQ=255) atatCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGACACGGCAATGCGGCGGcc > 2:34291/1‑150 (MQ=255) aGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTgcc < 2:161705/150‑1 (MQ=255) gCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGTGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc < 2:217784/150‑1 (MQ=255) gcgcATCGTGCTGGCACGGCCCCTGCTCAGCGGCCCCCTGGGCAACTGGCAGGCCGAGGAACAGAAGGTCCTGCAGGAGTTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTg < 2:63359/150‑1 (MQ=255) aTCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGc > 2:220499/1‑150 (MQ=255) caggcGGCCGCTGAGCAGCGGCCGTCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCt > 1:9439/5‑150 (MQ=255) aggcGGCCGCTGAGCAGCGGCCGTCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggag > 2:206740/4‑66 (MQ=25) aggcGGCCGCTGAGCAGCGGCCGTCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggag < 1:206740/63‑1 (MQ=25) ccGCGGCTCACGGGCCGCCTGGGCGACTGGCAGGCCGGGGGACAGAAGGTGCTGAAGGAGGCCACCGCCGCGCGCAAGGTGCCACGGTAGTGGGGCGGCCCGCCTTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCggg < 2:455860/150‑1 (MQ=255) gctgctCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGt > 2:137783/1‑150 (MQ=255) | TCAGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGGGT > NC_002947/4042219‑4042510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |