| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,672,980 | T→G | 18.5% | Q25H (CAA→CAC) | PP_4136 ← | LuxR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,672,980 | 0 | T | G | 18.5% | 45.7 / 8.7 | 27 | Q25H (CAA→CAC) | PP_4136 | LuxR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (9/13); new base G (2/3); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01 | |||||||||||
GAATGGCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGC > NC_002947/4672832‑4673129 | gAATGGCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACtt > 1:193871/1‑150 (MQ=255) gATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtgg > 2:259665/1‑146 (MQ=255) aTCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGt < 2:293757/150‑1 (MQ=255) aTCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtggt < 1:259665/150‑6 (MQ=255) cacaGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTgg > 2:534001/1‑150 (MQ=255) cacaGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtggtgt < 1:263909/150‑8 (MQ=255) cagcagCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCg > 1:614750/1‑150 (MQ=255) cagcCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACCTGGCGTCa < 1:119650/150‑1 (MQ=255) gTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCggg < 1:430350/150‑1 (MQ=255) aCTTCAAATTTGCGCGGGATGTTGGATATGTTAAAATGCACCGTTGTCTCCTTGCATTCAAGGATTTGGCCGATCTCGCACGAGCTATTCCCATAAGCACACAACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTcc < 2:25386/150‑1 (MQ=255) tCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtg < 2:36788/121‑4 (MQ=255) tCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCAAGTGCTGttgtg > 1:36788/1‑118 (MQ=255) aTTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAggg < 1:245491/150‑1 (MQ=255) gATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGa < 1:546898/150‑1 (MQ=255) tCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTg > 1:495281/1‑150 (MQ=255) gACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGaa > 2:119201/1‑150 (MQ=255) ccAAGGATCTGCCTGTTCTCCTACGCGCTTTTGGCATAAGCACACCACAACAGCATTTGCTGTTCACGTGGCGTCAGGCGAACCGGGTCGTCCGCCAGTGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTCAACTCCACGGGTGa < 2:193871/149‑1 (MQ=255) tGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCggg < 1:119201/150‑1 (MQ=255) tGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCggg > 1:402140/1‑150 (MQ=255) aCGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGTCGAACCggg > 1:352050/1‑65 (MQ=255) aCGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCggg < 2:352050/65‑1 (MQ=255) tctTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCa < 2:402140/150‑1 (MQ=255) acacCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTCCGaa < 2:614750/150‑1 (MQ=255) ccacAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGca > 2:524687/1‑150 (MQ=255) cacaACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGt < 2:525087/88‑1 (MQ=255) cacaACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGt > 1:525087/1‑88 (MQ=255) cacaACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGcac > 1:550484/1‑150 (MQ=255) aacaGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAgg < 2:302627/150‑1 (MQ=255) ttGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGc > 1:410320/1‑150 (MQ=255) | GAATGGCCTTGATCACAGCAGCCACCCGGGTAGGCACTTCAAATTTGCGCAGGATGTTGGATACGTGAAAATTCACCGTCGACTCCTTGCATTCAAGGATCTGGCCGATCTCCCACGAGCTCTTGCCATAAGCACACCACAACAGCACTTGCTGTTCACGTGGCGTCAGGCGAACCGGGGCGTCCGCCAGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGC > NC_002947/4672832‑4673129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |