Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,675,051 C→T 13.3% R593C (CGC→TGC)  PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,675,0510CT13.3% 71.4 / 2.8 30R593C (CGC→TGC) PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (16/10);  new base T (2/2);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCACCCTGTGGGAGCGCGCGGGCACTGGCAAGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCTGGGACTCACCGAAACAGGACT  >  NC_002947/4674906‑4675195
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cAGCACCCTGTGGGAGCGCGCGGGCACTGGCAAGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTa                                                                                                                                              <  1:446126/150‑1 (MQ=255)
  gCACCCTGTGGGAGCGCGCGGGCACTGGCAAGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATg                                                                                                                                            >  1:552224/1‑150 (MQ=255)
                cgcgcgGGCACTGGCAAGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGTGCTATGACTGGCCGAACGGc                                                                                                                              >  2:513007/1‑150 (MQ=255)
                                aGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGTGCTATGACTGGc                                                                                                                                      >  1:209877/1‑126 (MQ=255)
                                aGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGTGCTATGACTGGc                                                                                                                                      <  2:209877/126‑1 (MQ=255)
                                 gCTGAGCTTGCGCCACGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGCCaa                                                                                                             >  1:15564/1‑150 (MQ=255)
                                     aGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTTCCTTTATGACGACACGTTGGGCGACAACCTTCTGCCCGGCATCCCCATGCACTACTTCCAGTCCGTTCTGCTCTATGACTGGCCGAATGGCTTCTATGTCGTTGTCAATaca                                                                                                         <  2:434065/150‑1 (MQ=255)
                                             ccAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCTCGGCATCCCCATGCACCACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGc                                                                                                 <  2:73664/150‑1 (MQ=255)
                                             ccAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCTCGGCATCCCCATGCACCACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGc                                                                                                 >  1:73664/1‑150 (MQ=255)
                                                 gCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCg                                                                                             >  1:513628/1‑150 (MQ=255)
                                                    tACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTAt                                                                                                                                             >  2:115764/1‑99 (MQ=255)
                                                    tACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTAt                                                                                                                                             <  1:115764/99‑1 (MQ=255)
                                                       acCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTg                                                                                       <  2:513628/150‑1 (MQ=255)
                                                        ccTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGTGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGc                                                                                      <  1:513007/150‑1 (MQ=255)
                                                              gCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTgg                                                                                >  1:485534/1‑150 (MQ=255)
                                                                              tgatgaCGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTa                                                                >  2:312012/1‑150 (MQ=255)
                                                                                        acGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGAt                                                      >  2:338195/1‑150 (MQ=255)
                                                                                                 gACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAg                                                                                    <  1:403638/111‑1 (MQ=255)
                                                                                                 gACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAg                                                                                    >  2:403638/1‑111 (MQ=255)
                                                                                                 gACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAatat                                                <  1:321449/147‑1 (MQ=255)
                                                                                                 gACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAatat                                                >  2:321449/1‑147 (MQ=255)
                                                                                                           tGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGGCTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTg                                   >  2:444443/1‑150 (MQ=255)
                                                                                                              ccGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTgcgc                                >  2:25253/1‑150 (MQ=255)
                                                                                                               cGGCATCCCCATGCACTACTACCCGTGAGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTgcgcg                               >  1:84161/1‑150 (MQ=255)
                                                                                                                   aTCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGtt                           <  1:25253/150‑1 (MQ=255)
                                                                                                                       ccATGCACTACTACCAGGCCGAGCTGCGCTATGCCTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCCTTGGCt                       >  2:243520/1‑150 (MQ=255)
                                                                                                                       ccATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCt                       >  2:190742/1‑150 (MQ=255)
                                                                                                                                            aGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCTGGGACTCACCGAAACAGGACt  <  1:312012/150‑1 (MQ=255)
                                                                                                                                               tGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCTggg                    >  1:87349/1‑129 (MQ=255)
                                                                                                                                               tGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCTggg                    <  2:87349/129‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGCACCCTGTGGGAGCGCGCGGGCACTGGCAAGCTGAGCTTGCGCCAGGCCTACACCTTCAGCGACTTCCACTACCGTGATGACGACACGTTTGGCGACAACCGTCTGCCCGGCATCCCCATGCACTACTACCAGGCCGAGCTGCGCTATGACTGGCCGAACGGCTTCTATGCCGGGGTCAATACACAAATGGCCTCGAAGGTGCAGGTGGACTATGCCAATAGCTACCACGCCGATGAATATGCCTTGCTCGGTGCGCGCGTTGGCTGGGACTCACCGAAACAGGACT  >  NC_002947/4674906‑4675195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑