| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,732,554 | G→C | 23.5% | P85A (CCT→GCT) | sucB ← | 2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,732,554 | 0 | G | C | 23.5% | 23.2 / 5.2 | 17 | P85A (CCT→GCT) | sucB | 2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/7); new base C (2/2); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01 | |||||||||||
CCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCTCGAT > NC_002947/4732406‑4732692 | ccGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGgc > 1:269789/1‑150 (MQ=255) ttACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCCGCTGCGCCTGCCGCGGCAGCCGCacct > 2:64221/1‑147 (MQ=255) aCCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCCGCTGCGCCTGCCGCGGCAGCCGCacctgc < 1:64221/150‑6 (MQ=255) cGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGgcag > 2:567995/1‑150 (MQ=255) cAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGcccc > 2:166147/1‑149 (MQ=255) gCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATc < 2:349595/150‑1 (MQ=255) ttGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGgtg < 1:1709/150‑1 (MQ=255) tgagATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGAc < 2:228569/147‑1 (MQ=255) aTCGGGTCATCTTCGCCGGCGTCAGCGGCCGCAGCGGCCGGCGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTGGGACAGGACGGTGTCGCCCTCGCCCTTGACGATg > 1:343049/1‑150 (MQ=255) gggTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCg > 1:317338/1‑150 (MQ=255) cATCTTCGCCGGCGTCGGCGGCAGCAGCGGCCGGAGCAGCGCCAGGTTCGGCTGCCGCGGCACGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCa < 1:166147/150‑1 (MQ=255) cGTCAGCGGCAGCAGCGGCCGCAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCgg < 2:317338/150‑1 (MQ=255) cGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAg < 1:567995/150‑1 (MQ=255) gcTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAAcc > 2:396637/1‑150 (MQ=255) gctGCGCCTGCCGCGGCAGCCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCgg < 1:423365/116‑1 (MQ=255) gctGCGCCTGCCGCGGCAGCCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCgg > 2:423365/3‑118 (MQ=255) gCTGCCGCGGCAGGCGCAGCGGCAGCACCACCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCTCg > 1:562680/1‑150 (MQ=255) tGCCGGGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCTCGAt < 2:269789/150‑1 (MQ=255) | CCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGGACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCTCGAT > NC_002947/4732406‑4732692 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |