| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,837,326 | A→G | 14.8% | S430G (AGC→GGC) | ccoN‑I → | cbb3‑type cytochrome c oxidase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,837,326 | 0 | A | G | 14.8% | 61.3 / 4.0 | 27 | S430G (AGC→GGC) | ccoN‑I | cbb3‑type cytochrome c oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base A (10/13); new base G (2/2); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
CGCACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAACGCCT > NC_002947/4837186‑4837475 | cgcACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAg < 1:545343/150‑1 (MQ=255) gcACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAgg > 1:606105/1‑150 (MQ=255) caccaTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCtt > 1:203952/1‑150 (MQ=255) caTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTtcgt < 2:407945/150‑1 (MQ=255) caTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTtcgt < 2:32566/150‑1 (MQ=255) aTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCTGGCCAccaag > 1:183011/1‑127 (MQ=255) aTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCTGGCCACCAAg < 2:183011/130‑1 (MQ=255) tACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCTGGCCACCAAggt > 2:59743/1‑116 (MQ=25) tACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCTGGCCACCAAggt < 1:59743/117‑2 (MQ=25) tACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTc < 1:306276/150‑1 (MQ=255) ccTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCt > 2:89090/1‑145 (MQ=255) ccTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCt < 1:89090/145‑1 (MQ=255) cTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCgg > 1:249070/1‑150 (MQ=255) aCGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATgg > 1:169561/1‑150 (MQ=255) cGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCATCAGCGGCATGTTCCTGATGGCCTGGaa < 1:16847/150‑1 (MQ=255) ccAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAacac > 2:328218/1‑150 (MQ=255) gggCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTg < 2:203952/150‑1 (MQ=255) gCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGgc < 1:4550/150‑1 (MQ=255) gTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCa < 2:249070/150‑1 (MQ=255) aGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCtt < 1:328218/150‑1 (MQ=255) ggCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTc > 2:90863/1‑150 (MQ=255) gttcgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGc < 1:440729/113‑1 (MQ=255) gttcgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGc > 2:440729/1‑113 (MQ=255) ttcgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGt > 2:235833/1‑110 (MQ=255) ttcgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGt < 1:235833/110‑1 (MQ=255) tGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTgg < 1:90863/150‑1 (MQ=255) cTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTgagg > 1:361403/1‑150 (MQ=255) cagccaCCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAACGcc > 2:262038/1‑150 (MQ=255) agccaCCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCACCAGCGCTTGACGTCGCCCCTGTGAACGCCCAGTTGGCTTGAGGAGAAACGCCt < 2:606105/150‑1 (MQ=255) | CGCACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTCGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAACGCCT > NC_002947/4837186‑4837475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |