| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,889,791 | A→T | 17.1% | K14* (AAG→TAG) ‡ | glxR → | 2‑hydroxy‑3‑oxopropionate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,889,791 | 0 | A | T | 17.1% | 49.9 / 5.5 | 24 | K14* (AAG→TAG) ‡ | glxR | 2‑hydroxy‑3‑oxopropionate reductase |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base T (2/2); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGTTGCCCAGGAAGCCGAATTCATCATCGTC > NC_002947/4889645‑4889940 | ggCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGc < 2:359577/150‑1 (MQ=255) tGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAg < 1:363614/150‑1 (MQ=255) gAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCa > 2:458902/1‑150 (MQ=255) ccGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCGCATGGCTAAAATCGGTTTCATCGGCACCCGACTAATACGGCTTCCCATTGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTc > 2:173635/1‑150 (MQ=255) gCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg > 1:173786/1‑129 (MQ=255) gCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg < 2:173786/129‑1 (MQ=255) gCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGAAGGTCACAGCCTGTTCATTTcca > 1:62652/1‑150 (MQ=255) cacaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCTTGCCCATgatg > 2:324230/1‑102 (MQ=255) cacaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCTTGCCCATgatg < 1:324230/105‑4 (MQ=255) cacaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccacccac < 2:103628/150‑1 (MQ=255) ccAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGAcgcc > 2:600493/1‑150 (MQ=255) tCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCACCATGGGCTTGCCCATga < 2:250242/75‑2 (MQ=255) tCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCACCATGGGCTTGCCCATga > 1:250242/1‑74 (MQ=255) cttACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCCCGACGCCGCGCCAGCCGACCTGATCGCTGcc < 1:173635/149‑1 (MQ=255) aTGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGTCAGGTCACAGCCTGTTCATTTCCACCCACCTCGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAAc < 2:62652/150‑1 (MQ=255) tAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCCGCAAATGCAACAAGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGCTGCCGTGGCCCTGGCCAACCCGaa > 2:152354/1‑150 (MQ=255) tAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGaa > 2:152678/1‑150 (MQ=255) tCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg < 1:277999/50‑1 (MQ=255) tCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg > 2:277999/1‑50 (MQ=255) cGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGt < 1:152678/150‑1 (MQ=255) aTCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGTTGCCCAg > 1:123144/1‑150 (MQ=255) ccGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACCGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGa < 2:87821/96‑1 (MQ=255) ccGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGa > 1:87821/1‑96 (MQ=255) cGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACAACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGTTGCCCAGGAAGCCGa > 1:41824/1‑150 (MQ=255) aaGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGTTGCCCAGGAAGCCGAATTCATCATCGTc > 2:123816/1‑150 (MQ=255) | GGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTGGCCAACCCGAAAGAGGTTGCCCAGGAAGCCGAATTCATCATCGTC > NC_002947/4889645‑4889940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |