| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,992,076 | A→T | 25.0% | intergenic (‑183/‑16) | bkdR ← / → bkdAA | Bkd operon transcriptional regulator/branched‑chain alpha‑keto acid dehydrogenase complex subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,992,076 | 0 | A | T | 25.0% | 19.8 / 6.4 | 16 | intergenic (‑183/‑16) | bkdR/bkdAA | Bkd operon transcriptional regulator/branched‑chain alpha‑keto acid dehydrogenase complex subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (7/5); new base T (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
CTCAGCAAAGGCAATCAACTTGAGAGAAAAATTCTCCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCAACGATGCAGGTCAAGCCCGTAAACCCGCGATCGA > NC_002947/4991945‑4992213 | cTCAGCAAAGGCAATCAACTTGAGAGAAAAATTCTCCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATg < 2:285334/150‑1 (MQ=255) aGCAAAGGCAATCAACTTGAGAGAAAAATTCTCCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAAc > 1:549893/1‑150 (MQ=255) gagagaAAAATTCTCCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGt < 2:549893/150‑1 (MQ=255) tcCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTGCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAACGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCg > 1:140828/1‑150 (MQ=255) tAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCt < 2:140828/150‑1 (MQ=255) acaAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTg > 1:364475/1‑150 (MQ=255) caAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCCGGCTGCCAGACCGATTTTTCCTACCTgc > 2:111034/1‑150 (MQ=255) gTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCaa < 2:515674/150‑1 (MQ=255) tACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGTGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCAtgt > 1:158792/1‑92 (MQ=255) tACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGTGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCAtgt > 1:158793/1‑92 (MQ=255) tACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGTGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCAtgt < 2:158792/92‑1 (MQ=255) tACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGTGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCAtgt < 2:158793/92‑1 (MQ=255) aGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCAACGATGc > 1:489889/1‑150 (MQ=255) gcggagGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTCCGTTTGCATGTGCCCGCGCCCACCGGCCGGCCAGGCTCCCAGACCCATTTTTCCTACCTCCGCCTCAACGATGCa < 2:23578/150‑1 (MQ=255) aaaCAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCAACGATGCAGGTCAAGCCCGTAAACCCGcgatcg > 1:509932/1‑150 (MQ=255) aaCAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCAACGATGCAGGTCAAGCCCGTAAACCCGcgatcga > 1:330410/1‑150 (MQ=255) | CTCAGCAAAGGCAATCAACTTGAGAGAAAAATTCTCCTGCCGTGCCACTAAGATGTAGGTGACGCTGACTTACCAGTCACAAGCCGGTACTCAGCGGCGGCCGCTTCAGAGCTCACAAAAACAAATACCCGAGCGAGCGTAAAAAGCATGAACGAGTACGCCCCCCTGCGTTTGCATGTGCCCGAGCCCACCGGCCGGCCAGGCTGCCAGACCGATTTTTCCTACCTGCGCCTCAACGATGCAGGTCAAGCCCGTAAACCCGCGATCGA > NC_002947/4991945‑4992213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |