| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,075,430 | A→G | 12.9% | intergenic (+146/+174) | PP_4468 → / ← PP_t58 | Cro/CI family transcriptional regulator/tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,075,430 | 0 | A | G | 12.9% | 68.5 / 3.9 | 31 | intergenic (+146/+174) | PP_4468/PP_t58 | Cro/CI family transcriptional regulator/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base A (13/14); new base G (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01 | |||||||||||
ATCAGGCACAAAGGCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGGCAGCAAACCTGGTGCTTCAAA > NC_002947/5075284‑5075577 | aTCAGGCACAAAGGCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGcc < 2:592686/150‑1 (MQ=255) ggCACAAAGGCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAg > 1:80708/1‑150 (MQ=255) aaGGCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGtt < 1:116999/150‑1 (MQ=255) gaggACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAag < 2:184356/148‑1 (MQ=255) gCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCATGCACATTCCAGGAAAGTGCCGGGCGAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAag < 1:383008/150‑1 (MQ=255) gACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAgg > 2:565682/1‑150 (MQ=255) aaaCGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTagaa > 1:50913/1‑150 (MQ=255) tAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTagaagagaa < 2:359292/150‑1 (MQ=255) aTCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGcc < 2:579827/119‑1 (MQ=255) aTCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGcc > 1:579827/1‑119 (MQ=255) aTTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAAt > 2:281391/1‑150 (MQ=255) aCTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATg < 1:524163/118‑1 (MQ=255) aCTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATg > 2:524163/1‑118 (MQ=255) aTCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCAt < 1:439918/150‑1 (MQ=255) cAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAg < 1:565682/150‑1 (MQ=255) aaCGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAg > 1:246805/1‑150 (MQ=255) cGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGaa > 2:273972/1‑147 (MQ=255) cGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGaa < 1:273972/147‑1 (MQ=255) ttGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCg < 2:80708/150‑1 (MQ=255) cAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGata > 1:491949/1‑150 (MQ=255) tAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAg > 1:28204/1‑91 (MQ=255) tAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAg < 2:28204/91‑1 (MQ=255) tGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGGAGACTAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACACAACTCAAGCAAAGCGGATATGAAGAAcaca > 1:111987/1‑150 (MQ=255) gTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGgcag > 2:436586/1‑150 (MQ=255) acatgcttCGGCTGATCGGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGGCAGCaa > 2:528035/7‑150 (MQ=255) ttCGGCTGATCGGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCgaga > 2:570134/1‑80 (MQ=255) ttCGGCTGATCGGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCgaga < 1:570134/80‑1 (MQ=255) ttCGGCTGATCGGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGGCAGCAAACCTgg < 1:528035/150‑1 (MQ=255) tCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAAc > 2:73135/1‑79 (MQ=255) tCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGGCAGCAAACCTGGTGCTTCaaa < 2:246805/150‑1 (MQ=255) gcAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAAc < 1:73135/78‑1 (MQ=255) | ATCAGGCACAAAGGCGGACAAGCAAACGTAGATCAATTCCACTCTGCCCATCAATCGAACGACATCCATTTCCCGCAGCATCTCAGAAATTTTCCAGGAAATTGCCAGGCTAGGAATGAGCTGCTGGTAAGCGAGTTCGGCCGATCAGCCGAAGCATGTTAAGAGGCGTAGAAGAGAAGCAGAATAGCCCGACACCCATAGGAGAAGCGCCGAGAGCAGGAACTTCCACCCCACTCAACTCAAGCAAAGCGGATATGAAGAACACAGAGAAGGCAGCAAACCTGGTGCTTCAAA > NC_002947/5075284‑5075577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |