| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,079,174 | T→A | 22.2% | intergenic (‑371/+26) | mgtE ← / ← PP_t56 | magnesium transporter/tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,079,174 | 0 | T | A | 22.2% | 33.1 / 6.8 | 18 | intergenic (‑371/+26) | mgtE/PP_t56 | magnesium transporter/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base A (2/2); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01 | |||||||||||
CTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACT > NC_002947/5079028‑5079314 | cTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTAtt < 1:520269/150‑1 (MQ=255) gTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCgg < 1:216422/150‑1 (MQ=255) gttgtGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATg < 1:575997/150‑1 (MQ=255) gATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCTTTTTg < 1:167058/141‑1 (MQ=255) gATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCTTTTTg > 2:167058/1‑141 (MQ=255) aaaGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGa < 1:465878/150‑1 (MQ=255) aaaGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGa < 2:597893/150‑1 (MQ=255) acaTTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCTGACTcag < 2:392767/150‑1 (MQ=255) cacCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGAGCACCACCAAAAAGCCCCCTCTATTGAGCTCGCTATTGTTTAGGGAAGGGCTGAGTCTGCAGGAGTTCGAACCTGCGACCGCTCGGTTCGTAGCCGa > 2:531545/1‑150 (MQ=255) cGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAc > 1:268800/1‑150 (MQ=255) gagCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt > 2:205224/1‑150 (MQ=255) gATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCttttt < 1:254134/65‑1 (MQ=25) gATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCAATAGAGCGGGCttttt > 2:254134/1‑65 (MQ=25) aCAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTAATGGGGTATGGCGGACTAAGTAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGa < 1:200471/150‑1 (MQ=255) aTTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCa < 2:268800/150‑1 (MQ=255) acaaacaaAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAg > 1:30437/1‑143 (MQ=255) acaaacaaAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAg < 2:30437/143‑1 (MQ=255) gCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCGGCAGGATTTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACt < 1:531545/150‑1 (MQ=255) ctctATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTc < 2:106582/49‑1 (MQ=255) ctctATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTc > 1:106582/1‑49 (MQ=255) | CTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGATTTTTGAGGCCGCTCAGCGGCTCCAAACTACACCGCAATCATTACAGAAGAGCGTAGATCGACAGGCTCAAGAATTCATTGGCGCACAAACAAAAAGCCCGCTCTATTGAGCGGGCTTTTTGATGGGGTATGGCGGACTCAGCAGGA‑TTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGTGATGGTAGTTTTAGACCAGGTTACCACTGGCTTACT > NC_002947/5079028‑5079314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |