Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,182,972 G→C 9.2% I270M (ATC→ATG)  PP_4563 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,182,9720GC 9.2% 130.3 / 2.9 43I270M (ATC→ATG) PP_4563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGC  >  NC_002947/5182825‑5183112
                                                                                                                                                   |                                                                                                                                            
tGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGCAAGTCCTTTCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGAt                                                                                                                                            <  1:38267/150‑1 (MQ=255)
        gccgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCa                                                                                                                                    >  2:351673/1‑150 (MQ=255)
          cgcTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAgg                                                                                                                                  <  1:125313/150‑1 (MQ=255)
               caccaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                                                                                                      <  2:269683/141‑1 (MQ=255)
               caccaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                                                                                                      >  1:269683/1‑141 (MQ=255)
                accaTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGAGCGTCATGGATGACGATCAGGCCCTGc                                                                                                                            <  1:208525/150‑1 (MQ=255)
                  caTCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCtt                                                                                                                          >  2:117069/1‑150 (MQ=255)
                    tCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGCGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGa                                                                                                                        >  2:91959/1‑150 (MQ=255)
                        ccTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCGACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaacaa                                                                                                                    >  2:517902/1‑150 (MQ=255)
                               cTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGc                                                                                                                            <  1:307020/135‑1 (MQ=255)
                               cTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGc                                                                                                                            >  2:307020/1‑135 (MQ=255)
                                       cGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCctt                                                                                                     >  1:139597/1‑150 (MQ=255)
                                             ggACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                                                                                                      <  2:565979/111‑1 (MQ=255)
                                             ggACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGAt                                                                                                                                      >  1:565979/1‑111 (MQ=255)
                                                   gctgctGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGcc                                                                                         <  2:139597/150‑1 (MQ=255)
                                                              acacCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAAt                                                                                                           >  2:26067/1‑121 (MQ=255)
                                                              acacCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAAt                                                                                                           <  1:26067/121‑1 (MQ=255)
                                                                acCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTg                                                                            >  2:587619/1‑150 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaa                                                                                                                       >  1:425722/1‑105 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGaa                                                                                                                       <  2:425722/105‑1 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                          >  2:74723/1‑150 (MQ=255)
                                                                  ccGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGaa                                                                          >  2:611239/1‑150 (MQ=255)
                                                                       cGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCt                                                                     <  2:359771/150‑1 (MQ=255)
                                                                           tGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCt                                                                 <  2:303437/150‑1 (MQ=255)
                                                                             gCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                               <  1:611239/150‑1 (MQ=255)
                                                                             gCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                               <  1:74723/150‑1 (MQ=255)
                                                                                 gcagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCaa                                                           <  2:52759/150‑1 (MQ=255)
                                                                                  cagcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAAc                                                          <  1:117069/150‑1 (MQ=255)
                                                                                      ccgatctCCGCAACTTCGTACAGGCGTAAGGCCGTCAGTCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAg                                                      <  1:436486/144‑1 (MQ=255)
                                                                                              cGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAAGTGCCTTCTTGAAGTTGCCCGTGCGGACGGTGAACAGCTTGCTGAGCAACGCAggagatcgg                                              >  2:436486/1‑143 (MQ=255)
                                                                                                                tAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCAct                                               <  1:87628/131‑1 (MQ=255)
                                                                                                                tAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCAct                                               >  2:87628/1‑131 (MQ=255)
                                                                                                                  aGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                          <  1:28802/118‑1 (MQ=255)
                                                                                                                  aGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                          >  2:28802/1‑118 (MQ=255)
                                                                                                                   ggCCGTCAGGCTTGCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACAcc                         >  2:165973/1‑150 (MQ=255)
                                                                                                                              tttCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGc              >  1:338108/1‑150 (MQ=255)
                                                                                                                                 ctcGATGCGATCGTCATGGATGACGATCATGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTc           <  1:157526/150‑1 (MQ=255)
                                                                                                                                   cGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCa         <  1:517902/150‑1 (MQ=255)
                                                                                                                                        tgATCGTCATCCATGACGATCGGGCCCTGTTTGAACAACCCGCCAATGGCCTTCTTGaa                                                                                               <  2:289468/58‑1 (MQ=25)
                                                                                                                                        tgATCGTCATCCATGACGATCGGGCCCTGTTTGAACAACCCGCCAATGGCCTTCTTGaa                                                                                               >  1:289468/2‑59 (MQ=25)
                                                                                                                                        tgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCaa                             >  1:199554/2‑125 (MQ=255)
                                                                                                                                        tgATCGTCATCCATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCaa                             <  2:199554/124‑1 (MQ=255)
                                                                                                                                          aTCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGgcgcgc  >  1:323728/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TGGCAAGCGCCGCTCCACCATCAGCCTGGCCCTCAGCGCCGACCGGGACTCGCTGCTGCGCGACACCCGTCCGGGTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCACACCCCCAACACCCCGCCTTCGGCTTCCAGGCGCGC  >  NC_002947/5182825‑5183112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑