| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,217,251 | C→A | 12.3% | P92P (CCC→CCA) | moaA → | cyclic pyranopterin monophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,217,251 | 0 | C | A | 12.3% | 82.8 / 2.9 | 33 | P92P (CCC→CCA) | moaA | cyclic pyranopterin monophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base A (2/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
CAGCAAATCCTCAGCCTCGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAAGCAGCTGACCCGCACCGGCGAC > NC_002947/5217108‑5217398 | cAGCAAATCCTCAGCGTCGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCTCTGGGCAGCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCAGCGCCGCCCTGCCCGGCCTg > 2:37501/1‑150 (MQ=255) aTCCTCAGCCTCGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAg < 1:544018/150‑1 (MQ=255) ccTCGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGCGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCt > 2:464385/1‑150 (MQ=255) cGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGAc < 1:344778/150‑1 (MQ=255) cTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACAAGCAAc < 1:82270/150‑1 (MQ=255) ggTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCa > 2:397990/1‑150 (MQ=255) cGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCgg > 1:78574/1‑150 (MQ=255) cTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCt > 1:89876/1‑150 (MQ=255) gcTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCggg > 2:207299/1‑136 (MQ=255) gcTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCggg < 1:207299/136‑1 (MQ=255) cTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGc > 2:546163/1‑103 (MQ=255) cTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGc < 1:546163/103‑1 (MQ=255) cACCCGAAAGATCAGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGACTCGCAACTCGGGCGCCTGGCTCAGCCGCTGtt > 1:293065/1‑150 (MQ=255) ccGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGa > 1:433926/1‑150 (MQ=255) aTCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCggg < 2:169248/150‑1 (MQ=255) gCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGa > 2:192818/1‑150 (MQ=255) gCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCAGGCCTgc < 1:40223/78‑1 (MQ=255) gCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCAGGCCTgc < 1:39809/78‑1 (MQ=255) gCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCAGGCCTgc > 2:39809/1‑78 (MQ=255) gCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCAGGCCTgc > 2:40223/1‑78 (MQ=255) gggTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGCGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCt < 1:464385/150‑1 (MQ=255) gAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGAc > 1:202618/1‑126 (MQ=255) gAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGAc < 2:202618/126‑1 (MQ=255) ccAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCagcctggatagcct < 2:78574/150‑1 (MQ=255) aTCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGAAGCCGGGGTGGCACGCCTGAACATCAGCCTGGATAGCCTGGATGcc > 1:349223/1‑150 (MQ=255) ccgACCTGTGCCGGCGCACCCCCTCCCTCCCTGGACTGCGCGAGCTGTTCCTGACCAGTAACGGCCCGCAACTCGGGCGCCTGTTTCAGCCGCTGTTCTACGCCGGGGTGACACGCCTTAACATCAGCCTGGATAGCCTGGATGCCGAcc < 2:240752/149‑1 (MQ=255) aCCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGtt < 2:269046/94‑1 (MQ=255) aCCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGtt > 1:269046/1‑94 (MQ=255) cTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTc < 1:283105/150‑1 (MQ=255) tgCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAag > 1:303410/1‑150 (MQ=255) gCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAagc < 2:616581/150‑1 (MQ=255) cGGGCGCCTCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAagca < 1:183458/150‑1 (MQ=255) cccTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAAGCAGCTGACCCGCACCg > 2:92816/1‑150 (MQ=255) cccGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAAGCAGCTGACCCGCACCGGCGAc < 1:397990/150‑1 (MQ=255) | CAGCAAATCCTCAGCCTCGAAGAACTGTTCCAGGTGGCCGAACGCTTCGTCGCGCTGGGCACCCGAAAGATCCGCCTGACGGGTGGCGAGCCACTGGTACGCCAGGGCATCGTCGACCTGTGCGGGCGCATCGCCGCCCTGCCCGGCCTGCGCGAGCTGTGCCTGACCAGCAACGGCTCGCAACTCGGGCGCCTGGCTCAGCCGCTGTTCGACGCCGGGGTGACACGCCTGAACATCAGCCTGGATAGCCTGGATGCCGACCGTTTCAAGCAGCTGACCCGCACCGGCGAC > NC_002947/5217108‑5217398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |