| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,229,246 | C→G | 15.7% | intergenic (‑140/+36) | PP_4606 ← / ← PP_4607 | ferric siderophore receptor/transmembrane sensor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,229,246 | 0 | C | G | 15.7% | 93.0 / 9.7 | 37 | intergenic (‑140/+36) | PP_4606/PP_4607 | ferric siderophore receptor/transmembrane sensor |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base G (4/2); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
CTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGACCT > NC_002947/5229101‑5229394 | cTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑g > 1:611831/1‑150 (MQ=255) ttCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg < 2:611831/150‑1 (MQ=255) tGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTACACCCGCCCCTACGGG‑GGGttt > 2:233814/1‑150 (MQ=255) gTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACTCGTGAAGACGCTGGcaca > 2:343292/1‑150 (MQ=255) gTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGcaca > 2:464635/1‑150 (MQ=255) ttattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGCCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc < 1:514084/128‑1 (MQ=25) ttattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGCCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc > 2:514084/1‑128 (MQ=25) atcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTCCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAg > 1:419810/1‑150 (MQ=255) cACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAccc > 2:462218/1‑112 (MQ=255) cACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAccc < 1:462218/112‑1 (MQ=255) cACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAc < 2:602525/110‑1 (MQ=21) cACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAc > 1:602525/1‑110 (MQ=21) tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg > 2:54954/1‑101 (MQ=255) tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg < 1:54954/101‑1 (MQ=255) tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACTCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCaaa < 1:343292/150‑1 (MQ=255) aTTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCaa > 2:605730/1‑150 (MQ=255) aCCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCAt < 1:327060/150‑1 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAac < 2:466077/114‑1 (MQ=255) cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAac > 1:466077/1‑114 (MQ=255) aaTATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGaa < 1:612725/124‑1 (MQ=255) aaTATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGaa > 2:612725/1‑124 (MQ=255) tttttGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAgc < 1:165407/150‑1 (MQ=255) tgtgGCGGCCTCTTGGCGGGTAAACCCGCCCCTACGGG‑GCGTATACCCTGGAAGAGCTCCGGACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcagca > 2:483667/1‑150 (MQ=255) tgtgGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGcc > 2:510982/1‑93 (MQ=255) tgtgGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcagca < 1:605730/150‑1 (MQ=255) tgtgGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGATGGCACAGGCAACTACCGAACCAGGGTCAAACGcc < 1:510982/93‑1 (MQ=255) cagcCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGc > 1:400547/3‑150 (MQ=37) cgcgGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCAcc < 1:464635/150‑1 (MQ=255) gcgGGTAAACCCGCCCTTACGGG‑GGGTCTACGAGTGAAGAGGCTGACTCCGGCACCTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAGACCCATCACCTTGCCCAGCGAAGCCAGCGCCAGCAGCGGCTCGGCCACCg < 1:233814/150‑1 (MQ=255) gggTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCggg > 2:518136/1‑150 (MQ=255) gTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGaa < 1:518136/150‑1 (MQ=255) gTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGaa > 2:14812/1‑150 (MQ=39) tAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAg > 2:548078/1‑150 (MQ=255) tAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGAAGCGGCTCGGCAACCGGGAAg > 1:182041/1‑150 (MQ=255) ccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGcc > 2:48969/1‑150 (MQ=255) cGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCg < 1:548078/150‑1 (MQ=255) aCGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGACCt > 2:382707/1‑150 (MQ=255) | CTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGACCT > NC_002947/5229101‑5229394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |