| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,383,225 | G→A | 20.0% | P35L (CCG→CTG) | pdhR ← | DNA‑binding transcriptional regulator LldR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,383,225 | 0 | G | A | 20.0% | 34.5 / 4.8 | 20 | P35L (CCG→CTG) | pdhR | DNA‑binding transcriptional regulator LldR |
| Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (2/2); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TGAAGGTGGACCCCAACGACTCCGCCACAAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGG > NC_002947/5383082‑5383366 | tGAAGGTGAAACCCAACGCCTCCGCAACAAAATTTCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGcc < 2:247188/150‑1 (MQ=255) ccccAACGACTCCGCCACAAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGa < 1:191981/150‑1 (MQ=255) gACTCCGCCACAAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGc < 1:532214/150‑1 (MQ=255) cacaAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCAGCAGCCGCTGCCCCGAGGTCAGc < 1:33175/142‑1 (MQ=255) cacaAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCAGCAGCCGCTGCCCCGAGGTCAGc < 1:33221/142‑1 (MQ=255) cacaAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCAGCAGCCGCTGCCCCGAGGTCAGc > 2:33221/1‑142 (MQ=255) cacaAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCAGCAGCCGCTGCCCCGAGGTCAGc > 2:33175/1‑142 (MQ=255) caAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCa > 1:393127/1‑150 (MQ=255) tGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAatca > 2:267137/1‑150 (MQ=255) ccccGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATcc > 1:65413/1‑150 (MQ=255) cTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAg > 2:274128/1‑150 (MQ=255) cGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGa < 2:393127/150‑1 (MQ=255) gACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGAc < 1:267137/150‑1 (MQ=255) gACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGAc < 1:274128/150‑1 (MQ=255) cagcCCCTTGGCCACCAGATTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATgtc > 2:226853/1‑150 (MQ=255) accaGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGgcgc > 1:356953/1‑150 (MQ=255) ggcgggACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACAt < 2:65413/150‑1 (MQ=255) acCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCcaca > 1:377390/1‑150 (MQ=255) aaaCTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTCCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAAc < 2:377390/150‑1 (MQ=255) gcTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAggggg > 2:595081/1‑150 (MQ=255) cggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCaaa < 1:391895/133‑1 (MQ=255) cggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCaaa > 2:391895/1‑133 (MQ=255) ggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCa > 1:382841/1‑137 (MQ=255) ggcAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCa < 2:382841/137‑1 (MQ=255) | TGAAGGTGGACCCCAACGACTCCGCCACAAAATTGCCCCCGCCCTGACGGCTGACCAGCAGCCCCTTGGCCACCAGCTTCTGGATCGCCTCACGCAGTGACGGGCGGGACACACCAAACTGCTCGGCCAGGACGCGCTCGGCCGGCAGCCGCTGCCCCGAGGTCAGCGTGCCTTCCAGAATCATCCCTTCCAGCCGATCGACAATGTCGTCGGACAGGCGCCGTTGGCGTACCTGATCAAAAACCATCACATGCTCTCCACAAACCCCCGGCAAATTTCAGGGGG > NC_002947/5383082‑5383366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |