| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 431,365 | C→T | 12.5% | V318I (GTT→ATT) | PP_0354 ← | CBS domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 431,365 | 0 | C | T | 12.5% | 100.8 / 6.0 | 40 | V318I (GTT→ATT) | PP_0354 | CBS domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (15/20); new base T (3/2); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 | |||||||||||
GGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGTCA > NC_002947/431218‑431514 | ggTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCa < 1:306042/150‑1 (MQ=255) gCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCTTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGt > 2:21136/1‑150 (MQ=255) ccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCa < 2:327799/138‑1 (MQ=255) ccagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCa > 1:327799/1‑138 (MQ=255) cagTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATg < 2:61512/150‑1 (MQ=255) cGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTa < 1:210845/150‑1 (MQ=255) ggCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTgcgcg < 2:602980/150‑1 (MQ=255) ggCACACGGTATGGGCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCCTGGGGTATGGTgcgcg > 2:177454/1‑150 (MQ=255) aTGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAAc < 1:126470/150‑1 (MQ=255) tCGTTGCGCCACGTGATGAGCTGGGTGATCTGTGGGGACGATGAGCCATGTGCCAGGATGCGGTTGACGAGGTGGCTGAGTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGGACCAGa < 2:68198/150‑1 (MQ=255) aTGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAgg > 2:259365/1‑150 (MQ=255) aTGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAgg < 1:259365/150‑1 (MQ=255) gATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGga < 1:304159/150‑1 (MQ=255) aTCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGgag < 1:21136/150‑1 (MQ=255) tGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAAc < 1:489874/150‑1 (MQ=255) ggTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCa < 1:138395/125‑1 (MQ=255) ggTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCa > 2:138395/1‑125 (MQ=255) acgaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGaaa < 2:353239/150‑1 (MQ=255) gaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTc > 1:237223/1‑137 (MQ=255) gaTGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTc < 2:237223/137‑1 (MQ=255) ccATGGGCCAGCACGAGCAACACAAGCTGGCTGATTTCGCCGGCCAGCGAAACCAGCATATCCAGGCTTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGGTGCAGGGAGAAGAGGTCGCGCTCGGAAACCAcgccg > 2:6752/1‑150 (MQ=255) ggCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAg < 2:481808/150‑1 (MQ=255) ggCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAg < 2:397334/150‑1 (MQ=255) gCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAgg > 2:534866/1‑150 (MQ=255) gCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAgg > 2:295346/1‑150 (MQ=255) tGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGt > 1:454632/1‑150 (MQ=255) ttCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCAt > 2:254295/1‑59 (MQ=255) ttCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCAt < 1:254295/59‑1 (MQ=255) tACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTccac > 2:151403/1‑150 (MQ=255) atacgctgGTTTCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGc > 2:181494/5‑150 (MQ=255) tggctgATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAg > 2:60006/1‑150 (MQ=255) ggctgATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGa < 1:534866/150‑1 (MQ=255) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGa > 2:340965/4‑74 (MQ=37) tggtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGa < 1:340965/71‑1 (MQ=37) gtttCGCTGCGCGGCGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATgg > 2:263389/2‑150 (MQ=255) aTTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATgg < 2:454632/150‑1 (MQ=255) aTTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATgg < 1:295346/150‑1 (MQ=255) gcAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGAAGAAGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGcc > 1:315230/1‑150 (MQ=255) cGAAATCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTc < 1:181494/150‑1 (MQ=255) aaCCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGt > 2:170617/1‑150 (MQ=255) ccAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGAAGGGAGAACAGGTCGCCACTGGATACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGTca > 2:19298/1‑150 (MQ=255) | GGTCGCCACGTTCGGCCAGGGCCAGTTCGATGACCCGGCACACGGTATGGTCGTTGAGCAAGGTAATGATCTGGGTGATCTGCGTCGACGATGCGCCATGGGCCAGCATGCGTTCTACAAGCTGGCTGATTTCGCCGCGCAGCGAAACCAGCGTATCCAGGCGTGGCGCATGGCGTATGGTGCGCGCCAGGTGAACCAGATCGACCCGTTGCAGGGAGAACAGGTCGCGCTCGGAAACCACGCCGCACAGGCGTCCGTTTTCCACCAGGCAGACATGGGCAATATGCCGCTCGGTCA > NC_002947/431218‑431514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |