Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,015 A→G 15.6% F101S (TTC→TCC)  PP_0354 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,0150AG15.6% 68.9 / 6.3 32F101S (TTC→TCC) PP_0354CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (15/12);  new base G (2/3);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GACACCGCGCAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTC  >  NC_002947/431867‑432151
                                                                                                                                                    |                                                                                                                                        
gACACCGCGCAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGaa                                                                                                                                         >  2:193391/1‑150 (MQ=255)
                aaaTCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTg                                                                                                                         >  2:402129/1‑150 (MQ=255)
                      cGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCATTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTgg                                                                                                                           <  1:117032/142‑1 (MQ=255)
                      cGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCATTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTgg                                                                                                                           >  2:117032/1‑142 (MQ=255)
                        aaaCACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCCCTCCCCGCTGGTGATCTCGaa                                                                                                                 <  2:214752/150‑1 (MQ=255)
                                   aCATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGa                                                                                                      <  1:300905/150‑1 (MQ=255)
                                     aTCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCATGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGCTTCGaca                                                                                                    >  1:155882/1‑150 (MQ=255)
                                                  ggATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCa                                                                                       >  1:97487/1‑150 (MQ=255)
                                                       aaTGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGc                                                                                  <  1:555760/150‑1 (MQ=255)
                                                            ggCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGgcg                                                                             >  2:212170/1‑150 (MQ=255)
                                                              ccTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTggca                                                                                                                         >  2:604307/1‑102 (MQ=38)
                                                              ccTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTggca                                                                                                                         <  1:604307/104‑3 (MQ=38)
                                                              ccTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCt                                                                           >  1:263051/1‑150 (MQ=255)
                                                              ccTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGAGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGt                                                                                              >  1:312155/1‑131 (MQ=255)
                                                              ccTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGAGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGt                                                                                              <  2:312155/131‑1 (MQ=255)
                                                                     cAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAAc                                                                    >  2:188297/1‑150 (MQ=255)
                                                                                agAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGAtctc                                                                                                                    >  1:392815/1‑91 (MQ=255)
                                                                                agAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGAtctc                                                                                                                    <  2:392815/91‑1 (MQ=255)
                                                                                agAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCt                                                         <  1:212170/150‑1 (MQ=255)
                                                                                 gAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTg                                                        <  1:402129/150‑1 (MQ=255)
                                                                                        tCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGc                                                       >  1:437687/1‑144 (MQ=255)
                                                                                        tCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGc                                                       <  2:437687/144‑1 (MQ=255)
                                                                                                ggCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGaa                                         <  1:193391/150‑1 (MQ=255)
                                                                                                   cAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTg                                      >  2:99666/1‑150 (MQ=255)
                                                                                                              tGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGcc                           >  1:16247/1‑150 (MQ=255)
                                                                                                                               cccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACg          >  1:237892/1‑150 (MQ=255)
                                                                                                                                 cagcagGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTGCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGAt        >  2:86931/1‑150 (MQ=255)
                                                                                                                                 cagcagGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTACAAAGTGCCTTGGGCACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGAt        <  2:16247/150‑1 (MQ=255)
                                                                                                                                    cagGGCTGCCAGCGGGAAGAACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCt     <  2:68707/150‑1 (MQ=255)
                                                                                                                                       ggCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTc  >  2:37152/1‑150 (MQ=255)
                                                                                                                                            ccAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCgg                             >  2:358616/1‑118 (MQ=255)
                                                                                                                                            ccAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCgg                             <  1:358616/118‑1 (MQ=255)
                                                                                                                                             cAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGc                            <  2:448618/118‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
GACACCGCGCAGGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTC  >  NC_002947/431867‑432151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑