Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,557,975	T→G	15.4%	intergenic (‑295/+36)	PP_4888 ← / ← purA	methyl‑accepting chemotaxis transducer/adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,557,975	0	T	G	15.4%	56.4 / 4.4	26	intergenic (‑295/+36)	PP_4888/purA	methyl‑accepting chemotaxis transducer/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (10/12);  new base G (2/2);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCACGCGCAGC  >  NC_002947/5557827‑5558124                                                                                                                                                     |                                                                                                                                                      gCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTtctc                                                                                                                                                      >  2:86104/1‑150 (MQ=255)  cTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCa                                                                                                                                                     <  2:326816/150‑1 (MQ=255)              ccTGCGCCCAACCATCGTTGTGTGCCCACATAGCAGCTGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGt                                                                                                                                         >  1:206190/1‑150 (MQ=255)               cTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTGAGAAGGGCCGCGGCGtt                                                                                                                                        <  1:318549/150‑2 (MQ=255)                               ttGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAgg                                                                                                                        >  2:420877/1‑150 (MQ=255)                                tgtgtgCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGc                                                                                                                       <  1:153013/150‑1 (MQ=255)                                tgtgtgCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGc                                                                                                                       >  2:42621/1‑150 (MQ=255)                                  tgtgCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAg                                                                                                                     <  1:42621/150‑1 (MQ=255)                                         acaTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGc                                                                                                              <  1:420877/150‑1 (MQ=255)                                         acaTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGc                                                                                                              >  1:170720/1‑150 (MQ=255)                                                aGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACggg                                                                                                       <  1:55610/150‑1 (MQ=255)                                                          cccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGc                                                                                                                       <  1:197177/124‑1 (MQ=255)                                                          cccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGc                                                                                                                       >  2:197177/1‑124 (MQ=255)                                                                  tACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGGCCTTCTCAGGGCCGCGGCGTCAGATCAGCATGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGtc                                                                                     >  1:527208/1‑150 (MQ=255)                                                                  tACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGCAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGtc                                                                                     <  2:357181/150‑1 (MQ=255)                                                                                 aaCGGGTGATTACGGGTGGATATTGGCCAGCATAGACATGCCCACCAATGAAAACGCCGCGGCCCTTCTCAGGGCTGCGGCGTCAGATCAGCTTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCggg                                                                      <  2:68430/150‑1 (MQ=255)                                                                                      tGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGt                                                                 >  2:328231/1‑150 (MQ=255)                                                                                                 tGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGt                                                      <  2:170720/150‑1 (MQ=255)                                                                                                     tatTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTGTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGAt                                                  >  1:360905/1‑150 (MQ=255)                                                                                                      atTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATc                                                 <  1:612825/150‑1 (MQ=255)                                                                                                       ttGGCCAGCACAGACATGCCCACACATTAAAACGCCGCGCCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAACGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCg                                                <  2:527208/150‑1 (MQ=255)                                                                                                         ggCCAGCACAGACATGCCCACAAATGAAAACGCCGTCTCCGTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGgc                                              <  2:206190/150‑1 (MQ=255)                                                                                                                      aTGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGct                                 >  1:289688/1‑150 (MQ=255)                                                                                                                          ccACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTc                             >  1:561144/1‑150 (MQ=255)                                                                                                                                    tgaCGCCGCGGCCCTGAGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgca                                                                                                >  2:116549/3‑73 (MQ=21)                                                                                                                                    tgaCGCCGCGGCCCTGAGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgca                                                                                                <  1:116549/71‑1 (MQ=21)                                                                                                                                        gccgcGGCCCTGAGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCTATGCGCTTGATGt               >  1:566447/1‑150 (MQ=255)                                                                                                                                                     tcAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCACGCGCAGc  >  1:480312/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCACGCGCAGC  >  NC_002947/5557827‑5558124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑