| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,042,395 | A→T | 13.4% | E92D (GAA→GAT) ‡ | PP_5295 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,042,395 | 0 | A | T | 13.4% | 72.4 / 5.0 | 30 | E92D (GAA→GAT) ‡ | PP_5295 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (16/10); new base T (2/2); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
GCCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT > NC_002947/6042246‑6042540 | gcCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGaa < 1:446718/150‑1 (MQ=255) gCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCATGCGCAAGGGCCTGTCACCCAGCAAGGTGGAATGCa > 2:78781/1‑150 (MQ=255) aGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcg > 1:513257/1‑150 (MQ=255) gCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGcc > 2:22290/1‑150 (MQ=255) ccTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTAGGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa > 2:141302/1‑150 (MQ=255) ccTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa < 2:175726/150‑1 (MQ=255) tGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTACGCGTGCCCGAAGCTCTGCGCGAACTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGGACCTTGCGCCTCAACGAAGACAGCAATg > 1:569229/1‑150 (MQ=255) gTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgc < 1:252931/121‑1 (MQ=255) gTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgc > 2:252931/1‑121 (MQ=255) tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATg > 2:74809/1‑150 (MQ=255) ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc > 2:272811/1‑141 (MQ=255) ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc < 1:272811/141‑1 (MQ=255) gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg < 1:16705/97‑1 (MQ=255) gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg > 2:16705/1‑97 (MQ=255) ccTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGcgcgccgc < 1:78781/150‑1 (MQ=255) cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTg > 2:345509/1‑150 (MQ=255) tGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg > 1:513468/1‑150 (MQ=255) gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc > 1:423367/1‑88 (MQ=255) gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc < 2:423367/88‑1 (MQ=255) tGCGCGACATCGAAGGCTGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGccgccgccg > 2:596699/1‑150 (MQ=255) cgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGa > 2:547809/1‑150 (MQ=255) gCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc > 1:608378/1‑150 (MQ=255) gtgtACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGa < 2:608378/150‑1 (MQ=255) aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg < 1:263254/87‑1 (MQ=255) aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg > 2:263254/1‑87 (MQ=255) ggCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCg > 2:581137/1‑150 (MQ=255) cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc > 1:545864/1‑150 (MQ=255) cgcCAGGGCCTGTCCCGCGCCAAGGTGGAATGCACCTTGCGCCTCCACGAAGACAGCAATGGCAAACCCCTGAAAGCGGACCATGAGCGCGCCTCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGccccc < 1:188718/150‑1 (MQ=255) gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAg < 1:101480/150‑1 (MQ=255) ggCAAGGTGGCATGCACCTTGCGCCTCAACGAATACAGCAATGGCAAACCGCTGAAAGTGGACCCTGTATCCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTg < 1:596699/150‑1 (MQ=255) tGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt < 1:404707/150‑1 (MQ=255) | GCCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT > NC_002947/6042246‑6042540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |