Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,042,397 G→C 12.9% C93S (TGC→TCC)  PP_5295 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,042,3970GC12.9% 82.3 / 4.8 31C93S (TGC→TCC) PP_5295hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/11);  new base C (2/2);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCATGGCCA  >  NC_002947/6042250‑6042545
                                                                                                                                                   |                                                                                                                                                    
gCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCATGCGCAAGGGCCTGTCACCCAGCAAGGTGGAATGCa                                                                                                                                                    >  2:78781/1‑150 (MQ=255)
       aGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcg                                                                                                                                             >  1:513257/1‑150 (MQ=255)
         gCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGcc                                                                                                                                           >  2:22290/1‑150 (MQ=255)
             ccTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTAGGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                       >  2:141302/1‑150 (MQ=255)
             ccTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                       <  2:175726/150‑1 (MQ=255)
                           tGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTACGCGTGCCCGAAGCTCTGCGCGAACTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGGACCTTGCGCCTCAACGAAGACAGCAATg                                                                                                                         >  1:569229/1‑150 (MQ=255)
                                   gTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgc                                                                                                                                              >  2:252931/1‑121 (MQ=255)
                                   gTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgc                                                                                                                                              <  1:252931/121‑1 (MQ=255)
                                                   tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATg                                                                                                 >  2:74809/1‑150 (MQ=255)
                                                        ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc                                                                                                     <  1:272811/141‑1 (MQ=255)
                                                        ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc                                                                                                     >  2:272811/1‑141 (MQ=255)
                                                          gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg                                                                                                                                               >  2:16705/1‑97 (MQ=255)
                                                          gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg                                                                                                                                               <  1:16705/97‑1 (MQ=255)
                                                             ccTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGcgcgccgc                                                                                       <  1:78781/150‑1 (MQ=255)
                                                                    cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTg                                                                                >  2:345509/1‑150 (MQ=255)
                                                                     tGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg                                                                               >  1:513468/1‑150 (MQ=255)
                                                                      gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                            >  1:423367/1‑88 (MQ=255)
                                                                      gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                            <  2:423367/88‑1 (MQ=255)
                                                                                 tGCGCGACATCGAAGGCTGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGccgccgccg                                                                   >  2:596699/1‑150 (MQ=255)
                                                                                     cgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGa                                                               >  2:547809/1‑150 (MQ=255)
                                                                                                gCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                                    >  1:608378/1‑150 (MQ=255)
                                                                                                   gtgtACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGa                                                 <  2:608378/150‑1 (MQ=255)
                                                                                                            aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg                                                                                                       >  2:263254/1‑87 (MQ=255)
                                                                                                            aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg                                                                                                       <  1:263254/87‑1 (MQ=255)
                                                                                                              ggCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCg                                      >  2:581137/1‑150 (MQ=255)
                                                                                                                 cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc                                   >  1:545864/1‑150 (MQ=255)
                                                                                                                    cgcCAGGGCCTGTCCCGCGCCAAGGTGGAATGCACCTTGCGCCTCCACGAAGACAGCAATGGCAAACCCCTGAAAGCGGACCATGAGCGCGCCTCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGccccc                                <  1:188718/150‑1 (MQ=255)
                                                                                                                                    gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAg                <  1:101480/150‑1 (MQ=255)
                                                                                                                                      ggCAAGGTGGCATGCACCTTGCGCCTCAACGAATACAGCAATGGCAAACCGCTGAAAGTGGACCCTGTATCCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTg              <  1:596699/150‑1 (MQ=255)
                                                                                                                                             tGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt       <  1:404707/150‑1 (MQ=255)
                                                                                                                                                  tGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCATGGCCa  <  2:513468/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCATGGCCA  >  NC_002947/6042250‑6042545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑