| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,130,169 | G→C | 16.7% | A107G (GCC→GGC) | PP_5378 ← | metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,130,169 | 0 | G | C | 16.7% | 52.8 / 6.5 | 24 | A107G (GCC→GGC) | PP_5378 | metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (9/11); new base C (2/2); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
ATCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTC > NC_002947/6130022‑6130311 | aTCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCa > 1:531621/1‑150 (MQ=255) ggCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTg < 2:531621/150‑1 (MQ=255) aTTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGc < 1:366695/150‑1 (MQ=255) tttGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCg < 1:194606/150‑1 (MQ=255) tCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTgg > 1:437958/1‑142 (MQ=255) tCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTgg < 2:437958/142‑1 (MQ=255) aaGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAAAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGGGGGGGCGGTGCCgg > 1:281236/1‑150 (MQ=255) aaGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATaaa < 2:562704/150‑1 (MQ=255) aGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTACCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAg < 1:486545/150‑1 (MQ=255) aCAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCt < 1:81452/150‑1 (MQ=255) gCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCaa > 2:420528/1‑150 (MQ=255) gATACCGTGTTTAATTGTCCAGAACGCAGCTGCGGGGTCATCGATTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTa < 2:484568/150‑1 (MQ=255) ccGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTa > 2:572600/1‑150 (MQ=255) aactgctgGGTCTTCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGt > 2:289948/3‑150 (MQ=255) cGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATaaa > 2:111405/1‑51 (MQ=255) cGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATaaa < 1:111405/51‑1 (MQ=255) aTCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCAACGGCACAGATAAGGGTTTAATCCTTTAg > 2:269586/1‑150 (MQ=255) cTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCTCTGATAAGGGTTTCATCCTTTAGGTTc < 1:420528/150‑1 (MQ=255) ttAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCtt > 2:390728/1‑142 (MQ=255) ttAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCtt < 1:390728/142‑1 (MQ=255) aCCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAAc > 2:350937/1‑150 (MQ=255) ccAATCTTGCCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATc > 2:140091/1‑134 (MQ=255) ccAATCTTGCCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATc < 1:140091/134‑1 (MQ=255) aaTCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTc < 1:572600/150‑1 (MQ=255) | ATCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTC > NC_002947/6130022‑6130311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |