| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 510,145 | A→T | 12.1% | E54D (GAA→GAT) | trpD → | anthranilate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 510,145 | 0 | A | T | 12.1% | 90.7 / 3.0 | 33 | E54D (GAA→GAT) | trpD | anthranilate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (13/16); new base T (2/2); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CCGTATCGTCGGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGCTTCGTCCTTCGTCCTGGCG > NC_002947/510007‑510289 | ccGTATCGTCGGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGCGGGCCCCAAACGGGCCTTCCTTAGTGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTcggtgc > 1:228654/1‑150 (MQ=255) tATCGTCGGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCACCGACGATTTCGTcgatgcttt > 1:365659/1‑147 (MQ=255) aTCGTCGGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCACCGACGATTTCGTcgatgctttc < 2:365659/150‑5 (MQ=255) tcgGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCACCGACGATTTCGTcgatgcttt < 1:202042/145‑4 (MQ=255) tcgGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCACCGACGATTTCGTcgatgcttt > 2:202042/1‑142 (MQ=255) gggCAGTTGGACCTGACTAGAGAAGAAATGACAGAGGTCATGCTCCAGATCATGACCTGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTg > 2:315866/1‑150 (MQ=255) gggCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTg < 2:179288/150‑1 (MQ=255) gCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGAACCAGCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGAt < 1:469364/150‑1 (MQ=255) aGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGc < 1:2455/150‑1 (MQ=255) cTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGt < 2:512958/144‑1 (MQ=255) cTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGt > 1:512958/1‑144 (MQ=255) tACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGa > 2:488989/1‑150 (MQ=255) ccGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGaaa > 2:176117/1‑150 (MQ=255) gaagaaATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGaaaaa > 1:71844/1‑149 (MQ=255) aagaaATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAgg < 1:488989/150‑1 (MQ=255) gcgAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGc < 1:455975/150‑1 (MQ=255) aTGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAg > 1:424297/1‑120 (MQ=255) aTGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAg < 2:424297/120‑1 (MQ=255) gACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGt > 1:372415/1‑103 (MQ=255) gACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGt < 2:372415/103‑1 (MQ=255) aCCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGat < 1:9998/150‑1 (MQ=255) aGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCg < 2:228654/150‑1 (MQ=255) gTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGAATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCgg < 1:315866/150‑1 (MQ=255) ctgATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGAt > 2:471632/3‑150 (MQ=255) gATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATgg < 1:471632/150‑1 (MQ=255) ggCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGcc < 2:267731/150‑1 (MQ=255) aTGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGACGAGCTGCAAAACCTCGATCGTGTCGTCGATATTGTATGGACCGGTCGTGATGTCGCCTAAATCTTCAAc > 2:61793/1‑150 (MQ=255) gAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGcttcgtccttc < 1:176117/150‑1 (MQ=255) cATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGcttcgtccttc < 2:592367/145‑1 (MQ=255) cATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGcttcgtccttc > 1:592367/1‑145 (MQ=255) gacgaAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCaa < 2:216165/119‑1 (MQ=255) gacgaAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCaa > 1:216165/1‑119 (MQ=255) gacgaAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGCTTCGTCCTTCGTCCTggcg > 1:371404/1‑150 (MQ=255) | CCGTATCGTCGGGCAGTTGGACCTGACTACCGAAGAAATGCGCGAGGTCATGCGCCAGATCATGACCGGTCAGTGCAGCGAGGCGCAGATAGGCGCTTTCCTGATGGGCATGCGCATGAAAAGCGAAAGCATCGACGAAATCGTCGGTGCGGTGTCGGTGATGCGTGAGCTGGCCGAAAAGGTCGAGCTGCAAAGCCTCGATGGTGTCGTCGATATTGTCGGGACCGGTGGTGATGGCGCCAACATCTTCAACGTGTCCACCGCTTCGTCCTTCGTCCTGGCG > NC_002947/510007‑510289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |