| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 539,877 | C→T | 25.1% | A751V (GCG→GTG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 539,877 | 0 | C | T | 25.1% | 18.7 / 4.9 | 16 | A751V (GCG→GTG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (5/7); new base T (2/2); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
TGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCAT > NC_002947/539736‑539988 | tGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTgg > 1:243840/1‑150 (MQ=255) taggtaCCGGCATGGAGCGCAACGTTGCCCGTGACTGCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGGCGACGAAGTCGAAACCGGCGAAGCGGGTGTGGata > 2:29786/1‑150 (MQ=255) gtaCCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCt < 1:603567/150‑1 (MQ=255) gCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGAAGCGATCTGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGTGGGTGTGGATATCTACAAcc > 1:107547/1‑150 (MQ=255) tGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGa < 1:293420/150‑1 (MQ=255) gcgcAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCaa < 2:586579/150‑1 (MQ=255) tGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCa > 2:348236/1‑150 (MQ=255) gtgtGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGTGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGa < 2:107547/150‑1 (MQ=255) cGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCa < 2:92530/150‑1 (MQ=255) cGTATCGTTGTTCGCGTTTCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCCACAACCTGACCAAGTACACCCGTTCGAACCAGAGCACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCgtggt < 2:243840/150‑1 (MQ=255) ccGACGACGAAGTGGGAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGAcatcat < 2:132965/139‑1 (MQ=255) ccGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGAcatcat > 1:132965/1‑139 (MQ=255) aaCCGGCGAAGTGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACaa < 1:95256/102‑1 (MQ=255) aaCCGGCGAAGTGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACaa > 2:95256/1‑102 (MQ=255) ccGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCa < 1:556357/106‑1 (MQ=255) ccGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCa > 2:556357/1‑106 (MQ=255) | TGGTAGGTACCGGCATGGAGCGCAACGTTGCCCGTGACTCCGGTGTCTGCGTGGTTGCTCGCCGCGGTGGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCAT > NC_002947/539736‑539988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |