| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 648,250 | A→T | 12.5% | intergenic (+16/+75) | acoR → / ← accC | acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 648,250 | 0 | A | T | 12.5% | 84.4 / 5.2 | 32 | intergenic (+16/+75) | acoR/accC | acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base A (16/12); new base T (2/2); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
GTTGCTGGACGCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCT > NC_002947/648105‑648388 | gTTGCTGGACGCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGAcgcg > 2:188578/1‑150 (MQ=255) ttGCTGGACGCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCgg > 2:321849/1‑150 (MQ=255) gACGCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCTCTGGGGCCGCGTCGCGGCCCCAg < 1:492468/150‑1 (MQ=255) ccgatGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGAtt > 2:310300/5‑150 (MQ=255) ggAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAACCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAAc < 2:185946/150‑1 (MQ=255) tGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGccctg > 1:56300/1‑146 (MQ=255) gggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGttt < 2:56300/150‑2 (MQ=255) tttGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg > 2:92334/1‑149 (MQ=255) tttGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg < 1:92334/149‑1 (MQ=255) tGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTAc > 1:533286/1‑150 (MQ=255) ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCTCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACAt > 2:475870/1‑150 (MQ=255) gCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATc > 1:177956/1‑150 (MQ=255) cGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGAt < 1:326528/150‑1 (MQ=255) tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGt > 2:12418/1‑150 (MQ=255) cGAGTGCATGAGCACCGCATCGACATGCCTCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGc < 1:475870/150‑1 (MQ=255) cATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATAACACTACACGATCACTCCTGGTTGGACAGttt > 1:101247/1‑150 (MQ=255) cACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa > 2:223972/1‑108 (MQ=255) cACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa < 1:223972/108‑1 (MQ=255) aCCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCg > 1:525008/1‑150 (MQ=255) ccGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgt > 2:381250/1‑144 (MQ=255) ccGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgt < 1:381250/144‑1 (MQ=255) gCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAgg < 1:438943/150‑1 (MQ=255) aTCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTa < 2:525008/150‑1 (MQ=255) tCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAg < 1:12418/150‑1 (MQ=255) cATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGaa < 2:177956/150‑1 (MQ=255) cgcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTg > 1:362577/1‑150 (MQ=255) tAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCa < 1:321849/150‑1 (MQ=255) agaatcTGGGGCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGa > 2:585864/6‑150 (MQ=255) agCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa > 1:362790/1‑150 (MQ=255) cTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCt > 2:97210/1‑150 (MQ=255) cgcgTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa < 1:491188/64‑1 (MQ=255) cgcgTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa > 2:491188/1‑64 (MQ=255) | GTTGCTGGACGCGCTGATCCGCCATCGCTGGAAGCCGGCTGATGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCT > NC_002947/648105‑648388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |