Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 865,409 A→C 10.0% V140V (GTT→GTG)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947865,4090AC10.0% 99.6 / 5.1 40V140V (GTT→GTG) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (19/17);  new base C (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

TCGTTCAGGCGCTGGTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCGGGAT  >  NC_002947/865263‑865558
                                                                                                                                                  |                                                                                                                                                     
tCGTTCAGGCGCTGGTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGGGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAAcc                                                                                                                                                    >  1:195919/1‑150 (MQ=255)
  gTTCAGGCGCTGGTCGATCGAGGCGGTGTAGATCTTGACGTCCGGGTTGGCTTTTTACACAAACTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGACCGTTTTCAGCATGTCGCTGGTGTCAAcact                                                                                                                                                  >  2:107926/1‑147 (MQ=255)
  gTTCAGGCGCTGGTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTGCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCAt                                                                                                                                                  <  2:349374/150‑1 (MQ=255)
          gcTGGTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCGGCTCGATGGTTGCCACCATCGacctgc                                                                                                                                          <  1:181680/150‑6 (MQ=255)
              gTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGt                                                                                                                                      >  1:89961/1‑150 (MQ=255)
               tcgatcgaGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTg                                                                                                                                     >  1:418026/1‑150 (MQ=255)
                        gCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATc                                                                                                                            >  2:323970/1‑150 (MQ=255)
                               aGATCTTCACGTCCGTGTGTGCTTTTTCCCCCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCga                                                                                                                     <  2:233984/150‑1 (MQ=255)
                                    ttCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATc                                                                                                                <  2:404160/150‑1 (MQ=255)
                                      cACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAg                                                                                                              >  2:73632/1‑150 (MQ=255)
                                        cGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAggg                                                                                                            <  2:96341/150‑1 (MQ=255)
                                         gTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGc                                                                                                           <  1:73632/150‑1 (MQ=255)
                                                    cTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGt                                                                                                >  2:216212/1‑150 (MQ=255)
                                                     tttttCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGtt                                                                                               >  2:39115/1‑150 (MQ=255)
                                                           caccacCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTc                                                                                         >  2:568811/1‑150 (MQ=255)
                                                              caccTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCgg                                                                                      >  2:607282/1‑150 (MQ=255)
                                                                          ttCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTc                                                                          >  1:420512/1‑150 (MQ=255)
                                                                                     cgACCAGCACCATGGCGAGAATATCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTAGCCAGCATCGGGTCGATAAACAAATGCAGACCCGGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGgtgt                                                               <  1:568811/150‑1 (MQ=255)
                                                                                       aCCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGCTCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTgg                                                             <  1:323970/150‑1 (MQ=255)
                                                                                       aCCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTgg                                                             <  1:108796/150‑1 (MQ=255)
                                                                                         cAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTTGCCACCATCGacc                                                                                                                                             >  2:293581/1‑66 (MQ=38)
                                                                                         cAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTTGCCACCATCGacc                                                                                                                                             <  1:293581/68‑3 (MQ=38)
                                                                                           gCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCtt                                                         >  2:434589/1‑150 (MQ=255)
                                                                                                aTGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAgg                                                    <  2:418026/150‑1 (MQ=255)
                                                                                                   gcgcGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGtt                                                 >  2:285320/1‑150 (MQ=255)
                                                                                                     gcgAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGtttt                                               <  1:607282/150‑2 (MQ=255)
                                                                                                      cgAATCTCTTTGCAGCCGGCCTTTGTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTttct                                              <  1:434589/150‑1 (MQ=255)
                                                                                                        aaTCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCCGCTTGTGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTttcttc                                            <  1:39115/150‑1 (MQ=255)
                                                                                                               ttGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCgg                                     <  1:216212/150‑1 (MQ=255)
                                                                                                                          ccttttCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCt                                                          <  2:126256/116‑1 (MQ=255)
                                                                                                                          ccttttCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCt                                                          >  1:126256/3‑118 (MQ=255)
                                                                                                                          cTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGAc                          >  2:13602/1‑150 (MQ=255)
                                                                                                                                   cagGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCAAGCATAAGGCCGCAGATGAGGTCCGGGCTCTGGTTGCTGGCCTGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGAc                 <  1:139409/150‑1 (MQ=255)
                                                                                                                                      ctCGATGGTTGCCACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGACAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCtt              >  1:579832/2‑150 (MQ=255)
                                                                                                                                          aTGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGcc          >  2:388179/1‑150 (MQ=255)
                                                                                                                                          aTGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGcc          >  2:602783/1‑150 (MQ=255)
                                                                                                                                             gtggCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGccccc       >  1:354400/1‑150 (MQ=255)
                                                                                                                                               ggCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCgg     <  2:89961/150‑1 (MQ=255)
                                                                                                                                                gCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGccccc       <  1:590824/147‑1 (MQ=255)
                                                                                                                                                gCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGccccc       >  2:590824/1‑147 (MQ=255)
                                                                                                                                                  aaCCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCGGGAt  >  1:567736/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TCGTTCAGGCGCTGGTCGATCGAGGCGGTGTAGATCTTCACGTCCGGGTGGGCTTTTTCCACCACCTCGATGCCTTCTGGTGCGGCGACCAGCACCATGGCGCGAATCTCTTTGCAGCCGGCCTTTTTCAGCAGGTCGATGGTGGCAACCATCGAGCCGCCGGTGGCCAGCATCGGGTCGATGATCAGGGCCAGGCGCTGGTTGATGTCCGGCGCGAGCTTTTCCAGATAGGTGTGGGCTTCGAGGGTTTCTTCGTTTCGGGCAACGCCGACGGCGCTGACCTTGGCCCCCGGGAT  >  NC_002947/865263‑865558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑