| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,096,716 | G→C | 17.4% | P84R (CCG→CGG) | lptB ← | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,096,716 | 0 | G | C | 17.4% | 50.3 / 6.6 | 23 | P84R (CCG→CGG) | lptB | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (9/10); new base C (2/2); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
TTGTCCCGGATGTGGCTGATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCA > NC_002947/1096569‑1096834 | ttGTCCCGGATGTGGCTGATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGc > 1:289281/1‑150 (MQ=255) ccGGATGTGGCTGATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTa < 1:117356/150‑1 (MQ=255) cTGATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCg < 1:214866/150‑1 (MQ=255) gATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGc > 2:435326/1‑150 (MQ=255) tCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCCGCGGCAGGTAGCCGATTCCGGCACGGGCGCgg > 2:355688/1‑150 (MQ=255) cTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTG‑GGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCa < 1:85251/138‑1 (MQ=255) cTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTG‑GGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCa > 2:85251/1‑138 (MQ=255) tGCAGCAGGCTTTCCAGCGCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGACTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCg > 2:164794/1‑150 (MQ=255) gcagGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTACCTGCCGCAGGAAGcct < 1:576973/125‑2 (MQ=255) gcagGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTACCTGCCGCAGGAAGcct > 2:576973/1‑124 (MQ=255) tttCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCt > 2:248007/1‑150 (MQ=255) gCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTTCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGc < 1:454670/150‑1 (MQ=255) ctctTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCt < 1:123100/150‑1 (MQ=255) ccGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTg < 2:567831/150‑1 (MQ=255) aGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCCGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTAcc < 1:355688/150‑1 (MQ=255) ggTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTAccc < 2:310829/150‑1 (MQ=255) cTTGCGGGTCTCGCGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGcc > 2:473484/1‑150 (MQ=255) gggTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTACCTGCCGCAGGAAGc > 1:484008/1‑75 (MQ=255) gggTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTACCTGCCGCAGGAAGc < 2:484008/75‑1 (MQ=255) tcGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGcc < 1:572684/150‑1 (MQ=255) tcGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGcc > 2:572684/1‑150 (MQ=255) cgcggATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCt < 1:473484/147‑1 (MQ=255) cgacAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATGCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGATGATCTCGTGGGTGAGCATTAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCAGGTAGAAgga > 1:534555/1‑148 (MQ=255) | TTGTCCCGGATGTGGCTGATGTGGAACTCCTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCA > NC_002947/1096569‑1096834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |