Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,441 T→G 12.1% S293A (TCG→GCG)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4410TG12.1% 76.4 / 3.7 33S293A (TCG→GCG) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base T (11/18);  new base G (2/2);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

CGGAAGTCGCCGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAC  >  NC_002947/1121292‑1121587
                                                                                                                                                     |                                                                                                                                                  
cGGAAGTCGCCGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGACGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGt                                                                                                                                                    >  1:553133/1‑150 (MQ=255)
     gTCGCCGAGGAAGTGACCGAGCGCGAAGCGCTCCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCgg                                                                                                                                               <  2:49505/150‑1 (MQ=255)
           gAGGAAGTGACCGAGCTCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAAc                                                                                                                                         <  1:432580/150‑1 (MQ=255)
                 gTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGACGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCcgcga                                                                                                                                   <  2:553133/150‑2 (MQ=255)
                         gcgcGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCC‑CTGGCGCGGGTTGACCCGCGACAgcggcac                                                                                                                          <  1:389990/150‑7 (MQ=255)
                                gcgcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTc                                                                                                                    <  1:297414/150‑1 (MQ=255)
                                  gcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCaa                                                                                                                  <  1:300524/150‑1 (MQ=255)
                                    ttCTCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGCCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                <  1:112567/150‑1 (MQ=255)
                                    ttCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTTTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgc                                                                                                                >  1:468671/1‑150 (MQ=255)
                                       ccACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCa                                                                                                                   >  2:94083/1‑144 (MQ=255)
                                       ccACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCa                                                                                                                   <  1:94083/144‑1 (MQ=255)
                                         aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGctgctg                                                                                                           <  1:151330/150‑1 (MQ=255)
                                            tCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCg                                                                                                        <  1:116485/150‑1 (MQ=255)
                                                        ggCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTg                                                                                            >  1:5065/1‑150 (MQ=255)
                                                            aggaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACa                                                                                        <  2:20160/150‑1 (MQ=255)
                                                                                      cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTcc                                                              >  1:277306/1‑150 (MQ=255)
                                                                                       tGCAATGGCGGTTGTCGCTGCCCATCCTGGTGCTCGTCCTCACGCGGGTAGCGGTGCCTCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCg                                                             <  1:35669/150‑1 (MQ=255)
                                                                                        gCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGt                                                                                                                                             <  1:425113/69‑1 (MQ=255)
                                                                                        gCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGt                                                                                                                                             >  2:425113/1‑69 (MQ=255)
                                                                                          aaTGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTAc                                                          >  1:312291/1‑150 (MQ=255)
                                                                                                  ttGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGcc                                                  >  2:183692/1‑150 (MQ=255)
                                                                                                     tCGCTGCCCATCCTGGTGTTCGTCCTCACCCTTCTCGCTGTTCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTc                                               <  2:468671/150‑1 (MQ=255)
                                                                                                                      gtTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTCCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGcc                              <  2:340119/150‑1 (MQ=255)
                                                                                                                          gtcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATc                          <  2:277306/150‑1 (MQ=255)
                                                                                                                          gtcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATc                          >  1:413484/1‑150 (MQ=255)
                                                                                                                          gtcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCGGCGGTGCGTGGCCTACCTGACAATGCTGATTTCCGTAAAAGGAGGCCTCGACAATGGCAAACGGCCGATc                          >  1:136638/1‑150 (MQ=255)
                                                                                                                           tcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCg                         <  1:183692/150‑1 (MQ=255)
                                                                                                                               cACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCt                     >  1:325991/1‑150 (MQ=255)
                                                                                                                                 cccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGTCAAACTGCCGATCGCCCTgg                   <  1:215479/150‑1 (MQ=255)
                                                                                                                                           ggTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCg                                                                                                        <  2:606776/55‑1 (MQ=255)
                                                                                                                                           ggTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCGTCCTCAAGCTGCTGCCg                                                                                                        >  1:606776/1‑55 (MQ=255)
                                                                                                                                                 ccTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                            <  2:261355/124‑1 (MQ=255)
                                                                                                                                                  cTGGCGCGGGTTGACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGa                            >  2:523795/1‑124 (MQ=255)
                                                                                                                                                  cTGGCGCGGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAc  >  1:257604/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGAAGTCGCCGAGGAAGTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAC  >  NC_002947/1121292‑1121587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑