Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 96,580 C→T 10.8% Q35Q (CAG→CAA)  PP_0088 ← alkanal monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294796,5800CT10.8% 93.8 / 3.7 37Q35Q (CAG→CAA) PP_0088alkanal monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (17/16);  new base T (2/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

CCCCGCCAGAGCCCACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGATTGGCCAGAGGCCTTTTT  >  NC_002947/96441‑96721
                                                                                                                                           |                                                                                                                                             
ccccGCCAGAGCCCACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGt                                                                                                                                     >  2:584411/1‑150 (MQ=255)
     ccAGAGCCCACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGc                                                                                                                                >  2:84903/1‑150 (MQ=255)
          gCCCACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGtt                                                                                                                           <  2:527919/150‑1 (MQ=255)
            ccACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTgc                                                                                                                         <  1:84903/150‑1 (MQ=255)
              aCGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTgcgc                                                                                                                       >  2:559074/1‑150 (MQ=255)
              aCGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTgcgc                                                                                                                       >  2:392865/1‑150 (MQ=255)
               cgcgAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCa                                                                                                                      <  1:86703/150‑1 (MQ=255)
                      aCGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTg                                                                                                               <  1:470878/150‑1 (MQ=255)
                          gAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGcgcg                                                                                                           <  1:392865/150‑1 (MQ=255)
                             gTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCgggg                                                                                                        <  1:584411/150‑1 (MQ=255)
                                cccccGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAg                                                                                                                                       >  2:250710/1‑116 (MQ=255)
                                cccccGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAg                                                                                                                                       <  1:250710/116‑1 (MQ=255)
                                cccccGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCACAGCGCTTGCGCGGGGCCa                                                                                                     >  1:28688/1‑150 (MQ=255)
                                  cccGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCAAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGc                                                                                                   <  1:608531/150‑1 (MQ=255)
                                      gCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGCGTTCCGCAGCGATTGCGCGGGGCCAGCGTCg                                                                                               <  1:420245/150‑1 (MQ=255)
                                       ccAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCgg                                                                                              >  1:158236/1‑150 (MQ=255)
                                              gCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTAc                                                                                       <  1:122491/150‑1 (MQ=255)
                                                      gCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCa                                                                               >  1:316983/1‑150 (MQ=255)
                                                        aCCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAgg                                                                             <  1:278779/150‑1 (MQ=255)
                                                                 gCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTg                                                                    <  2:158236/150‑1 (MQ=255)
                                                                  ccGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAgg                                                                                                                                      >  1:318682/1‑83 (MQ=255)
                                                                  ccGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAgg                                                                                                                                      <  2:318682/83‑1 (MQ=255)
                                                                          gCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCATGGTGGAAATCTTc                                                           <  1:417309/150‑1 (MQ=255)
                                                                                              gtgCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCAt                                       >  1:220670/1‑150 (MQ=255)
                                                                                                        aCCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCg                             >  2:283502/1‑150 (MQ=255)
                                                                                                          ccAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCa                           >  2:613552/1‑150 (MQ=255)
                                                                                                             gAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCaaaa                        <  1:559074/150‑1 (MQ=255)
                                                                                                                   gTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGAtt                  >  1:306451/1‑150 (MQ=255)
                                                                                                                    ttATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGATTg                 >  2:126782/1‑150 (MQ=255)
                                                                                                                          ccAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAg                                                         >  1:473263/1‑104 (MQ=255)
                                                                                                                          ccAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAg                                                         <  2:473263/104‑1 (MQ=255)
                                                                                                                              aGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGATTGGCCAGAGGcc       >  1:140223/1‑150 (MQ=255)
                                                                                                                                 gcTCGACATGTTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCaaa                         >  1:100599/1‑129 (MQ=255)
                                                                                                                                 gcTCGACATGTTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCaaa                         <  2:100599/129‑1 (MQ=255)
                                                                                                                                 gcTCGACATGTTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCaaa                         <  2:100455/129‑1 (MQ=255)
                                                                                                                                 gcTCGACATGTTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCaaa                         >  1:100455/1‑129 (MQ=255)
                                                                                                                                   tCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGATTGGCCAGAGGCCttttt  >  2:303306/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CCCCGCCAGAGCCCACGCGAATACGTGAGGTCCCCCCGGCCAGGTAGCCGATCAGCACCGCAGTCGCCGAACTGGCGATGCCGTCCATGTTGTGGTGCTCGGCCACCCAGAAGCGGTTATAGCCAAAGCGCTCGACATGCTGCGCCAGGTCCAGCGAGTTGCGCAGCGATTGCGCGGGGCCAGCGTCGGCGCGTACGGGCACCAGGTCCAGGGTGGAAATCTTCAGGTCACGCAGCTGCGTCATGGAGCCTCCGCAAAAGTGATTGGCCAGAGGCCTTTTT  >  NC_002947/96441‑96721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑