| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,606,449 | A→T | 17.4% | intergenic (‑157/+54) | PP_1407 ← / ← PP_t64 | sulfate transporter/tRNA‑Gly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,606,449 | 0 | A | T | 17.4% | 50.5 / 6.3 | 23 | intergenic (‑157/+54) | PP_1407/PP_t64 | sulfate transporter/tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base T (2/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
TATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTC > NC_002947/1606332‑1606586 | tatCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCtt > 2:294296/1‑127 (MQ=255)tatCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCTTGGCTCCtt < 1:294296/127‑1 (MQ=255) cGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCCTGGGCCAGGCCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGa > 1:607493/1‑117 (MQ=255) cGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGa < 2:607493/117‑1 (MQ=255) gcacgcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAAc < 2:402585/126‑1 (MQ=255) gcacgcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAAc > 1:402585/1‑126 (MQ=255) gcacgcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAg < 1:570914/150‑1 (MQ=255) gcaAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCggg > 2:309963/1‑150 (MQ=255) cccGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGAtgtg > 1:177966/1‑136 (MQ=255) cccGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGAtgtg < 2:177966/136‑1 (MQ=255) aTGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATc < 2:212518/150‑1 (MQ=255) aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAccc > 1:413740/1‑150 (MQ=255) aTTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAAccc > 1:213128/1‑150 (MQ=255) gATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTaaa > 1:3744/1‑150 (MQ=255) aTTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCtt > 2:224333/1‑142 (MQ=255) aTTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCtt < 1:224333/142‑1 (MQ=255) tGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGtttt < 2:413740/150‑1 (MQ=255) gTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTaa > 2:279953/1‑150 (MQ=255) ggATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATAccc < 1:309963/150‑1 (MQ=255) gATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCg > 1:27904/1‑150 (MQ=255) aaaaaaGGAGCCAAGGCTCCTTTTGACTGCGATGACGATGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTc < 1:523189/150‑1 (MQ=255) aaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCggg > 1:541417/1‑72 (MQ=255) aaaGGAGCCTTGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCggg < 2:541417/72‑1 (MQ=39) | TATCCGCCACAAGCCGTTTCGCTTGCGCACGCAAAGGCCCGATGCGCATTGAAGCGGATCGGTGGGTAATTGGCCATGGGCCAGGTCAAGGCAGGATGAAAAACGAAAAAAGGAGCCAAGGCTCCTTTTGACTGCGATGACGTTGCTGGAACGCCATGCGGGACTGCGATGTGGAGCGGGTAGAGGGAATCGAACCCTCAACTAAAGCTTGGGAAGCTTTCGTTTTGCCACTAAACTATACCCGCATCTGAGCTC > NC_002947/1606332‑1606586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |