| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,825,134 | G→C | 13.4% | A54A (GCC→GCG) | mutS ← | DNA mismatch repair protein MutS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,825,134 | 0 | G | C | 13.4% | 74.5 / 6.1 | 30 | A54A (GCC→GCG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base G (13/13); new base C (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
TCCACCGGGCCCTTGCTGGTGGCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCGGGGTGTGTGCG > NC_002947/1824985‑1825281 | tCCACCGGGCCCTTGCTGGTGGCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGTTCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCTCACATCGGGATGGACTGGCCGGCCGACTGACCgcgg < 2:131355/150‑1 (MQ=255)tCCACCGGGCCCTTGCTGGTGGCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCgcgg > 1:513845/1‑150 (MQ=255) cTTGCTGGTGGCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCCCGCGGTCAgtcg < 2:168351/150‑4 (MQ=255) ggCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCcag < 2:223237/150‑1 (MQ=255) cGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCcagca < 1:155546/150‑1 (MQ=255) gTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGttt > 1:590544/1‑150 (MQ=255) tCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTc < 2:590544/150‑1 (MQ=255) cGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCgg < 1:579295/150‑1 (MQ=255) caccGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTa > 1:405089/1‑150 (MQ=255) aCTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTagaaga < 2:513845/150‑1 (MQ=255) aCTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTagaaga < 1:246854/150‑1 (MQ=255) ccGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCg < 2:405089/150‑1 (MQ=255) gTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGaa > 2:583239/1‑150 (MQ=255) tAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAAc > 2:267215/1‑150 (MQ=255) tGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCggg < 1:267215/150‑1 (MQ=255) aaCGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGc > 2:425915/1‑150 (MQ=255) aaTCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGtt > 1:215640/1‑150 (MQ=255) cccGCACATGGGGAGGAACTCGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTANAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGttttt < 1:21322/150‑1 (MQ=255) ccGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGttttca > 1:285007/1‑148 (MQ=255) cGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCa > 1:130838/1‑150 (MQ=255) caTGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGG‑TTTTCAGCTTc < 2:285007/150‑1 (MQ=255) aTGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTc < 1:149552/150‑1 (MQ=255) aTGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCCGGTGCTGGTTTTTCAGCTTc > 2:590404/1‑150 (MQ=255) ggATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGt > 1:538990/1‑150 (MQ=255) ggACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTActg < 1:405202/150‑1 (MQ=255) gACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGGCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCggg > 1:294388/1‑150 (MQ=255) gACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGt < 1:465689/112‑1 (MQ=255) gACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGt > 2:465689/1‑112 (MQ=255) gACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCggg > 2:509549/1‑150 (MQ=255) ccTGACCGCCCGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTAc < 2:400272/133‑1 (MQ=255) ccTGACCGCCCGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTAc > 1:400272/2‑134 (MQ=255) ccTGACCGCCCGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCgggg > 1:540451/2‑150 (MQ=255) cgggcggTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCGGGGTGTGTGCg < 2:538990/150‑1 (MQ=255) | TCCACCGGGCCCTTGCTGGTGGCGGGGTCGCCGATCTGCTCACAGATCACCACCGACTCGCCGAGCTTGACCAGCTTGGCCAGGTAGCCTTCCAGCGAATGGAACGGAATCCCGCACATGGGGATGGACTGGCCGGCCGACTGACCGCGGGCGGTCAGGGTGATATCCAGCAGTTTCGCGGCCTTTTTCGCATCTTCGTAGAAGATCTCGTAGAAGTCGCCCATGCGGTAGAACATCAGCTGGTCCGGGTGCTGGTTTTTCAGCTTCCAGTACTGCTGCATCATCGGGGTGTGTGCG > NC_002947/1824985‑1825281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |