Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,852,047	C→A	100%	A364D (GCC→GAC)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,852,047	0	C	A	100.0%	53.3 / NA	17	A364D (GCC→GAC)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/11);  total (6/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGA  >  NC_002947/1851908‑1852185                                                                                                                                            |                                                                                                                                           ttCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGgcgc                                                                                                                                  >  1:354272/1‑150 (MQ=255)        acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAgg                                                                                                                           <  1:573468/150‑1 (MQ=255)                                  ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAc                                                                                                 >  2:537797/1‑150 (MQ=255)                                        cgcgcgTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTAc                                                                                           <  1:377694/150‑1 (MQ=255)                                                gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGACAATCCCAGCTCCAACCACTACGAAGaaaa                                                                                   <  1:476423/150‑1 (MQ=255)                                                        cGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGTCCCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCCCTACGAAGAAAAGATTTCCt                                                                           <  2:125345/150‑1 (MQ=255)                                                                     cGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGt                                                              <  2:354272/150‑1 (MQ=255)                                                                                      aGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTg                                                          <  1:278995/137‑1 (MQ=255)                                                                                      aGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTg                                                          >  2:278995/1‑137 (MQ=255)                                                                                           cAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCa                                                                 <  2:278591/125‑1 (MQ=255)                                                                                           cAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCa                                                                 >  1:278591/1‑125 (MQ=255)                                                                                                     accACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAg                                                                                                                            >  1:468353/2‑56 (MQ=255)                                                                                                     accACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAg                                                                                                                            <  2:468353/55‑1 (MQ=255)                                                                                                            cATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcct                       >  1:363233/1‑150 (MQ=255)                                                                                                               gCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctggc                    <  2:363233/150‑1 (MQ=255)                                                                                                                               cTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc    <  1:537797/150‑1 (MQ=255)                                                                                                                                 tGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGa  <  1:434233/150‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           TTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGA  >  NC_002947/1851908‑1852185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑