| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,395,386 | G→C | 22.8% | G282R (GGC→CGC) ‡ | PP_2998 → | 2‑dehydropantoate 2‑reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,395,386 | 0 | G | C | 22.8% | 38.1 / 10.0 | 22 | G282R (GGC→CGC) ‡ | PP_2998 | 2‑dehydropantoate 2‑reductase |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base C (2/3); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01 | |||||||||||
GCTTGAGCCTGACCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGAGCCAGCCAAAAGCCTACCTCGAACACGTGGCGTTCTGG > NC_002947/3395243‑3395528 | gCTTGAGCCTGACCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAgc < 1:529427/150‑1 (MQ=255) gCCTGACCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTACCGCTGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATg > 1:112964/1‑150 (MQ=255) ccTGACCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGCCCCAgcgcacgac < 1:85416/150‑6 (MQ=255) aCCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTg < 2:556811/150‑1 (MQ=255) gCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAAc < 2:421701/150‑1 (MQ=255) gCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATc < 1:169580/150‑1 (MQ=255) cTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAgg > 2:505613/1‑150 (MQ=255) tGGAAAAAAGCTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAggg < 1:538753/150‑1 (MQ=255) ggAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGa > 1:306786/1‑150 (MQ=255) aaCTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCa < 2:112964/150‑1 (MQ=255) tCGTTCACCGGAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGa < 1:258391/150‑1 (MQ=255) cACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGagccag < 2:372629/150‑1 (MQ=255) aCCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGagccagc < 1:505613/150‑1 (MQ=255) aTCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGaaa < 2:601592/130‑1 (MQ=255) aTCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGaaa > 1:601592/1‑130 (MQ=255) aaCGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGAGCCAGCCAAAAGCCTACCTCGAac > 1:557838/1‑150 (MQ=255) catggcgcTGGCCCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCAt < 1:578583/110‑1 (MQ=255) catggcgcTGGCCCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCAt > 2:578583/5‑114 (MQ=255) cgtGCGCTGGGACCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGGCCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGAGCCAGCCAAAAGCCTACCTCGAACACGTGGCGTTc > 2:193848/1‑150 (MQ=255) tggcgcTGGCCCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCtggtg < 2:440914/52‑1 (MQ=37) tggcgcTGGCCCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCtggtg > 1:440914/3‑54 (MQ=37) gcgcTCGGGCCAGCGCCATGGCGTGGCCACGCCGGTCTACGCGTCCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGAGCCAGCCAAAAGCCTACCTCGAACACGTGGCGTTCTgg < 1:554981/150‑1 (MQ=255) | GCTTGAGCCTGACCAGCCCCGAGGCTGCCTGGTCACTGGCCGCCGAAGGCCTGCCGGCGAGCTTCCGCACCTCGATGCTGCAGAGCCTGGAAAAAAACTCGATCACCGAAATCGACTTCATCAACGGCTCCGTCGTGCGCTGGGGCCAGCGCCATGGCGTGGCCACGCCGGTCAACGCGACCCTGGTGGCCTGCATCAAGGGTATCGAGCGCGCCATGGCCGATCACCAGAAGAGGGAAACCCCATGAGCCAGCCAAAAGCCTACCTCGAACACGTGGCGTTCTGG > NC_002947/3395243‑3395528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |