| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,030,799 | C→T | 15.1% | intergenic (+37/‑458) | PP_4433 → / → dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,030,799 | 0 | C | T | 15.1% | 76.1 / 6.7 | 33 | intergenic (+37/‑458) | PP_4433/dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base T (2/3); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTC > NC_002947/5030654‑5030941 | tGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCa < 2:413018/150‑1 (MQ=255) actacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAg < 2:197253/150‑1 (MQ=255) catGGTTGCATCAGCACTTGGGATTTTTGCTTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTAGCACCCAGGCtctat < 1:252685/150‑5 (MQ=255) tCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCa > 2:234157/1‑150 (MQ=255) cTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTAc > 2:379367/1‑150 (MQ=255) ttGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGt < 1:379367/150‑1 (MQ=255) tgtgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCgg > 2:306643/1‑130 (MQ=255) tgtgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCgg < 1:306643/130‑1 (MQ=255) aTCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGAGCAAGCCGACCGGCTACGTCGata > 2:183509/1‑150 (MQ=255) gagaCTAAAGGTTTCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATaa < 2:340721/150‑1 (MQ=255) tAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGc < 1:126123/94‑1 (MQ=255) tAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGc > 2:126123/1‑94 (MQ=255) aaaGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcagca < 1:87363/150‑1 (MQ=255) aaaGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCT‑ATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcagcag > 1:34071/1‑150 (MQ=255) gATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTATTTCGTAGGCACATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCCCCACCCTTCCTTTTTa > 1:412787/1‑150 (MQ=255) aaTAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTgg > 1:195002/1‑150 (MQ=255) tAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTgg > 1:249652/1‑150 (MQ=255) tAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCATCCTTTTTATTGTTATCTCACCTCTTGGCATCTgg > 1:290548/1‑150 (MQ=255) tGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 2:195002/150‑1 (MQ=255) tGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 1:234157/150‑1 (MQ=255) tGGGTGCTGATTAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTa < 1:592907/101‑1 (MQ=255) tGGGTGCTGATTAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTa > 2:592907/1‑101 (MQ=255) tGGGTGCTGATTAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTa > 1:410336/1‑150 (MQ=255) gggTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTAt < 2:34071/150‑1 (MQ=255) tGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTAtctc > 2:541868/1‑150 (MQ=255) gCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAgcgc < 1:565566/79‑1 (MQ=255) gCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAgcgc > 2:565566/1‑79 (MQ=255) gCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAgcgc > 2:565405/1‑79 (MQ=255) gCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAgcgc < 1:565405/79‑1 (MQ=255) cTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTc < 1:277947/137‑1 (MQ=255) cTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTc > 2:277947/1‑137 (MQ=255) tAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACccttc < 1:272343/87‑1 (MQ=255) tAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACccttc > 2:272343/1‑87 (MQ=255) | TGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCAGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTC > NC_002947/5030654‑5030941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |