| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,557,779 | A→G | 11.5% | intergenic (‑99/+232) | PP_4888 ← / ← purA | methyl‑accepting chemotaxis transducer/adenylosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,557,779 | 0 | A | G | 11.5% | 91.4 / 3.5 | 37 | intergenic (‑99/+232) | PP_4888/purA | methyl‑accepting chemotaxis transducer/adenylosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base A (14/18); new base G (2/2); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
AAACACAGGCCGCAAGAGGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGT > NC_002947/5557630‑5557923 | aaacaCAGGCCGCAAGAGGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCaa < 1:234833/150‑1 (MQ=255) caGGCCGCAAGAGGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCt < 2:362477/150‑1 (MQ=255) gCAAGAGGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAAt > 2:442454/1‑150 (MQ=255) agagGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATggg < 1:215630/150‑1 (MQ=255) gcggcTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGatt > 1:392440/1‑150 (MQ=255) gcTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCgt < 2:76032/150‑1 (MQ=255) gTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTGTTCTGGCGCCACTATTTTGATTGCATCTACAGATACTTCTTACAGCAAGGTTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCa < 2:26811/150‑1 (MQ=255) tAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAg < 2:553117/150‑1 (MQ=255) aaCAGTGAGAGGATGGCAGACACGTTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAgg > 2:594129/1‑150 (MQ=255) gagaGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCATGCCCAtt < 2:392440/150‑1 (MQ=255) gtAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTa < 1:42403/150‑1 (MQ=255) tCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGc < 2:395546/150‑1 (MQ=255) gtTTTCTGGCGCCACTATTTTGATGGCATTTATAGAACGTTCTCACAGAAAGGGTTGTGCTGCAGGCAGCGTGGACTTGCCCCAAGGTTTAGCAATGGGGTTTCGC‑GTGCAGGCCCATTCGCGGGTGAACCCGTTCCTACAACGGCCttt < 2:418611/150‑3 (MQ=255) gcgcCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAAcc > 1:246957/1‑150 (MQ=255) gcCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCt < 2:455023/128‑1 (MQ=255) gcCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCt > 1:455023/1‑128 (MQ=255) aCTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTcgc > 2:441040/1‑109 (MQ=255) aCTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTcgc < 1:441040/109‑1 (MQ=255) cTATTTTGATCGCAGGTAGAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGtt > 2:305493/1‑150 (MQ=255) tttttGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTtgt < 2:553438/149‑1 (MQ=255) aTCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATa > 2:290184/1‑150 (MQ=255) tCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAg > 1:590120/1‑150 (MQ=255) tACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGAGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGa > 1:417210/1‑150 (MQ=255) aGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCg > 2:462873/1‑150 (MQ=255) gAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCgg < 2:590120/150‑1 (MQ=255) gAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGAGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCgg < 2:417210/150‑1 (MQ=255) aaaCTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGCCATGCGACATCGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCggg < 1:442454/150‑1 (MQ=39) ttcttACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTa > 1:118826/1‑150 (MQ=255) ttACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTAccc > 1:420165/1‑150 (MQ=255) aCAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCcgc < 1:462873/150‑1 (MQ=255) aGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTAACCCGCGa > 1:548412/1‑150 (MQ=255) gggTTGTGCCGCAGGCAGCGGGGAAATGCGCCAGGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACg < 1:400375/93‑1 (MQ=255) gggTTGTGCCGCAGGCAGCGGGGAAATGCGCCAGGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACg > 2:400375/1‑93 (MQ=255) gggTTGTGCCGCAGGCAGCGGGGAAATGCGCCAGGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACg > 2:400347/1‑93 (MQ=255) gggTTGTGCCGCAGGCAGCGGGGAAATGCGCCAGGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACg < 1:400347/93‑1 (MQ=255) tgCCGCAGGCAGCGGGGAAATGCGCCAACGTC‑ACCCACAGGATCTCGTCCAGCAGTTCCATTCGCGGGTCACACCGGTCGTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAgcg < 1:594129/150‑1 (MQ=255) tGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCg < 1:290184/150‑1 (MQ=255) cgcCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGt < 1:1154/150‑1 (MQ=255) | AAACACAGGCCGCAAGAGGCGGCTTCGTAACAGTGAGAGGATGGCAGACACGGTGTAACCCTCGTGTTTTCTGGCGCCACTATTTTGATGGCATCTACAGAAACTTCTTACAGCAAGGGTTGTGCCGCAGGCAGCGGGGAAATGCGCCAAGGTCTAGCAATGGGGTTTCGT‑GTGCAGGCCCATTCGCGGGTAAACCCGCTCCTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGT > NC_002947/5557630‑5557923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |