| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,610,627 | G→A | 13.3% | intergenic (‑62/‑10) | PP_4929 ← / → emrE | LysR family transcriptional regulator/small multidrug resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,610,627 | 0 | G | A | 13.3% | 69.9 / 4.2 | 30 | intergenic (‑62/‑10) | PP_4929/emrE | LysR family transcriptional regulator/small multidrug resistance protein |
| Reads supporting (aligned to +/- strand): ref base G (16/10); new base A (2/2); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
CGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCATTGCGTTCTGGATGC > NC_002947/5610479‑5610775 | cgcgGAGAATGATCGCAGTTCTGCAAAGCGTAGAAAGGTACCCAATTGTTCAAGTTTCCACTGCTCGGCCATCAACCTATCCCCCATATCGATCGGTAATGACTTTACTGCTGCCAGACAACGTCGAGATTGCCCGCTCAGTAACggagg < 2:159255/150‑1 (MQ=255) aGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATc > 2:320380/1‑142 (MQ=255) aGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATc < 1:320380/142‑1 (MQ=255) aGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGc > 1:603395/1‑150 (MQ=255) ttCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCt > 2:363422/1‑150 (MQ=255) cTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCa > 1:241583/1‑150 (MQ=255) ggCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCt > 1:504009/1‑150 (MQ=255) cATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAAGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGa > 2:505780/1‑150 (MQ=255) cATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAAGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGa > 2:202178/1‑150 (MQ=255) aaCCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGaa > 2:387066/1‑150 (MQ=255) tcCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGa > 1:299253/1‑150 (MQ=255) atCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGcc < 1:387066/150‑1 (MQ=255) tCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAAGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCt < 1:202178/150‑1 (MQ=255) tCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAAGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCt < 1:505780/150‑1 (MQ=255) gATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGa < 2:184731/150‑1 (MQ=255) tAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGc > 1:135525/1‑150 (MQ=255) tAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGATCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGc > 1:582187/1‑150 (MQ=255) aaTGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCtat < 2:363509/73‑1 (MQ=255) aaTGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCtat > 1:363509/1‑73 (MQ=255) cTTTACCCCATCCAGGCAACGTCTAGATTGCCCGCTCAGTAACGGAGATTCGTTCCACGAAGGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGc < 2:418384/150‑1 (MQ=255) ccccATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCActg > 1:568469/1‑150 (MQ=255) cccATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGaaa < 2:550331/125‑1 (MQ=255) cccATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGaaa > 1:550331/1‑125 (MQ=255) ccATCTAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCActgct > 1:309176/1‑150 (MQ=255) ccATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGc < 1:171006/143‑1 (MQ=255) ccATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGc > 2:171006/1‑143 (MQ=255) tGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATgg < 2:568469/150‑1 (MQ=255) ccGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCAt > 1:308896/1‑150 (MQ=255) cGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCAtt < 2:603395/150‑1 (MQ=255) tAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAATTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCATTGCGTTCt < 2:299253/150‑1 (MQ=255) aCGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCATTGCGTTCTgg > 1:476954/1‑150 (MQ=255) aggaTCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCATTGCGTTCTGGATGc < 1:363422/150‑1 (MQ=255) | CGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGGCCATCAACCTATCTCCCATATCGATAGGTAATGACTTTACCCCATCCAGGCAACGTCTAGAATGCCCGCTCAGTAACGGAGGATCCTGCCATGAATGCCTATACCTACCTCGCCATTGCCATCTGCGCCGAAGTCATCGCCACCGCCTCGATGAAGGCGGTGAAAGGCCTGAGCACGCCGTTGCCACTGCTGCTGATGGTGGTTGGCTATGGCATTGCGTTCTGGATGC > NC_002947/5610479‑5610775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |