| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 874,554 | T→C | 16.6% | Y44H (TAC→CAC) | PP_0759 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 874,554 | 0 | T | C | 16.6% | 53.0 / 3.5 | 24 | Y44H (TAC→CAC) | PP_0759 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/9); new base C (2/2); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCT > NC_002947/874408‑874699 | ggCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACa < 2:181393/150‑1 (MQ=255) ccTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATg > 2:382948/1‑150 (MQ=255) tAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGtt < 1:382948/150‑1 (MQ=255) gATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGa > 1:446341/1‑150 (MQ=255) ggAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAgg > 2:549346/1‑150 (MQ=255) tCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGa < 2:192202/150‑1 (MQ=255) cGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCTATGAGGTCAACCAGATGTAc < 2:296034/150‑1 (MQ=255) gTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTAcg > 2:223140/1‑150 (MQ=255) tCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAg > 1:558810/1‑150 (MQ=255) atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATcag > 1:366002/1‑150 (MQ=255) tgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg < 2:446341/150‑1 (MQ=255) gCTGGCATCCTGCGGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGTGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGaa < 1:526289/150‑1 (MQ=255) aTCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTACTGTTGGCG‑TTCGACGCCAACAGCAc < 2:280774/72‑1 (MQ=25) ccAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGa < 1:223140/150‑1 (MQ=255) aaTACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAgc > 1:504354/1‑150 (MQ=255) tACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAgcgc > 2:521341/1‑150 (MQ=255) caccaGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGa < 1:521341/150‑1 (MQ=255) caGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGc < 2:558810/150‑1 (MQ=255) tacTGTTGGCGTTCG‑ACGCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATc > 2:429930/3‑105 (MQ=38) tacTGTTGGCGTTCG‑ACGCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATc < 1:429930/103‑1 (MQ=38) tacTGTTGGCGTTCG‑ACGCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgtgt > 2:535623/3‑150 (MQ=255) tGTTGGCGTTCG‑ACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGATGTGCTCGACAAGTCCAGCGCCGATGCCACGCGTGTGcc > 2:80796/1‑149 (MQ=255) gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGcc > 1:130705/1‑150 (MQ=255) aCAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCt > 2:161242/1‑150 (MQ=255) | GGCCTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCG‑TCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCT > NC_002947/874408‑874699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |