| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,382,332 | A→T | 33.3% | intergenic (+12/+15) | dinB → / ← PP_1204 | DNA polymerase IV/lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,382,332 | 0 | A | T | 33.3% | 13.5 / 10.2 | 15 | intergenic (+12/+15) | dinB/PP_1204 | DNA polymerase IV/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (6/4); new base T (2/3); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTC > NC_002947/1382194‑1382457 | ttATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGTGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCtt < 1:304551/150‑1 (MQ=255) tATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTc < 2:460860/150‑1 (MQ=255) tCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTTGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTa > 2:216230/1‑150 (MQ=255) aaGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTg > 1:91742/1‑150 (MQ=255) gTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGCCCAGTGGCCttt > 2:462774/1‑149 (MQ=255) accggcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTg < 2:426259/146‑1 (MQ=255) taggggtaggggTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTg > 1:69408/1‑140 (MQ=255) taggggtaggggTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTg < 2:69408/140‑1 (MQ=255) gtaggggtGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTTGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAgg < 1:216230/150‑1 (MQ=255) tGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCct > 1:228972/1‑150 (MQ=255) aaCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATACGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCt > 1:186677/1‑150 (MQ=255) atagaaGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTc < 1:259747/137‑1 (MQ=255) atagaaGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTc > 2:259747/5‑141 (MQ=255) gaaGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg < 1:434506/114‑1 (MQ=255) gaaGGGGCTGCATTGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg > 2:434506/2‑115 (MQ=255) | TTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGCATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTC > NC_002947/1382194‑1382457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |