| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,436,102 | C→T | 13.8% | intergenic (‑132/+21) | PP_1256 ← / ← PP_1257 | alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,436,102 | 0 | C | T | 13.8% | 67.0 / 4.3 | 29 | intergenic (‑132/+21) | PP_1256/PP_1257 | alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (2/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
GTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGTCAGGACGTCGGTCTCGTTGAAGTGCAGGCTGTACTC > NC_002947/1435960‑1436248 | gtgAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGga > 2:201118/1‑150 (MQ=255) ggggCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGc < 1:161012/150‑1 (MQ=255) ggCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAg < 1:201118/150‑1 (MQ=255) aGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGgctgc > 1:77105/1‑150 (MQ=255) aTCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGttt > 2:65164/1‑150 (MQ=255) aTCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGttt < 1:363242/150‑1 (MQ=255) aTGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTc < 1:65164/150‑1 (MQ=255) ttACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATAcc < 2:228020/150‑1 (MQ=255) cGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATAc < 2:87829/146‑1 (MQ=255) cGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATAc > 1:87829/1‑146 (MQ=255) gACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctacatgcccctc < 2:56809‑M2/150‑63 (MQ=255) aCCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCTacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctacatgcccctca < 2:537141‑M2/150‑64 (MQ=255) cTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGa > 2:100291/1‑150 (MQ=255) cTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGa > 2:503011/1‑150 (MQ=255) gTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCacaggggtcgtaatcccccccatgcatctgcattagat < 1:106598‑M2/119‑39 (MQ=255) gTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCacaggggtcgtaatcccccccatgcatctgcattagat > 2:106598‑M2/1‑81 (MQ=255) gAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGAc > 1:250215/1‑122 (MQ=255) gAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGAc < 2:250215/122‑1 (MQ=255) gAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTTGTATGCCAGCTggtc < 2:303915/143‑4 (MQ=255) gAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGTATGCCAGCTggtc > 1:303915/1‑140 (MQ=255) gTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCGGTG‑TTCGCGGGTGAACCCGCTCCTacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctac < 1:44319‑M2/125‑54 (MQ=255) gTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCGG‑TGTTCGCGGGTGAACCCGCTCCTacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctac > 2:44319‑M2/1‑72 (MQ=255) tCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCTacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctacatgcccctcacaggcaacctgctgatcg > 1:537141‑M2/1‑69 (MQ=255) cGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCacaggggtcgtaatcccccccatgcatctgcattagatttccaaggaggctacatgcccctcacaggcaacctgctgatcggccagaggccggtaacc > 1:56809‑M2/1‑52 (MQ=255) gCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGa > 1:557281/1‑150 (MQ=255) tG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAAAGCCGACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGTCAGGACGTCGGTCTCg < 1:503011/150‑1 (MQ=255) gCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGt > 1:574749/1‑120 (MQ=255) gCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGt < 2:574749/120‑1 (MQ=255) tCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGTCAGGACGTCGGTCTCGTTGAAGTGCAGGCTGTACTc < 1:100291/150‑1 (MQ=255) | GTGAGGGGCATGTAGCCTCCTTGGAAATCTAATGCAGATGCATGGGGGGGATTACGACCCCTGTAGGAGCGGGTTCACCCGCGAACACCGGCGAAGCCGGTGCCATGCACCGGGTCGCCTG‑A‑TTCGCGGGCACGCCCGCTCCCACAGGGAGAGCAGTGGCTGCTCAGGCGATGTTTTGCTCGGCCGACCAGCTGGCATACCAGCTGCGGAACAGCGCGTACTGCTGTTCGGCATAGTTGCGCTGAGCGTCGGTCAGGACGTCGGTCTCGTTGAAGTGCAGGCTGTACTC > NC_002947/1435960‑1436248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |