BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,948,680	Δ1 bp	coding (213/435 nt)	fliO ←	flagellar protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,948,680	0	C	.	99.5%	940.0 / ‑2.6	193	coding (213/435 nt)	fliO	flagellar protein FliO
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (96/96); minor base G (0/1); total (96/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 193 total aligned reads displayed.

TGCCGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCT  >  NC_002947/4948536‑4948824

tGCCGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGc                                                                                                                                             <  2:4469803/150‑1 (MQ=255)

 gCCGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGcc                                                                                                                                            >  2:594469/1‑150 (MQ=255)

  ccGGCACTTCCACGGGTTCGGCCAGCACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGccc                                                                                                                                           >  2:2249879/1‑150 (MQ=255)

   cGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCa                                                                                                                                          >  1:3603619/1‑150 (MQ=255)

   cGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCa                                                                                                                                          >  1:4605846/1‑150 (MQ=255)

         ttCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTc                                                                                                                                    >  2:3701064/1‑150 (MQ=255)

          tCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCa                                                                                                                                   >  1:5172561/1‑150 (MQ=255)

            cACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACt                                                                                                                                 >  1:2000360/1‑150 (MQ=255)

             aCGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTg                                                                                                                                >  1:2924146/1‑150 (MQ=255)

                ggTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCgg                                                                                                                             <  1:3542377/150‑1 (MQ=255)

                ggTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCgg                                                                                                                             >  1:909002/1‑150 (MQ=255)

                ggTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCgg                                                                                                                             <  1:2862295/150‑1 (MQ=255)

                   tCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGc                                                                                                                          >  1:2488687/1‑150 (MQ=255)

                      gCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                       <  2:1227376/150‑1 (MQ=255)

                      gCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                       <  2:1893536/150‑1 (MQ=255)

                         aGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCa                                                                                                                    <  2:4636687/150‑1 (MQ=255)

                          gTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAg                                                                                                                   >  2:1406913/1‑150 (MQ=255)

                               tGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑Gcgcc                                                                                                             <  2:16025/150‑1 (MQ=255)

                                gCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCAGAGCACCGCAgtgaagg                                                                                                             <  2:207844/150‑7 (MQ=255)

                                 cAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑Gcgccgc                                                                                                           <  2:1866966/150‑1 (MQ=255)

                                  aGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCgca                                                                                                          <  1:2184579/150‑1 (MQ=255)

                                   gggCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGCGC‑CTGCAGAGCACCgcag                                                                                                         <  1:4868200/150‑1 (MQ=255)

                                     gCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGTCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCgcagca                                                                                                       <  1:3224345/150‑1 (MQ=255)

                                     gCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCgcagcc                                                                                                       <  2:2723448/150‑2 (MQ=255)

                                       cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCagcagc                                                                                                     <  2:745274/150‑1 (MQ=255)

                                       cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGCGC‑CTGCAGAGCACCgcagtgaa                                                                                                     <  2:616245/150‑5 (MQ=255)

                                        tCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgcc                                                                                                    <  2:2693590/150‑1 (MQ=255)

                                          gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgccag                                                                                                  <  1:370731/150‑1 (MQ=255)

                                          gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgccag                                                                                                  <  2:4867397/150‑1 (MQ=255)

                                          gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTGCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgccag                                                                                                  >  2:4765011/1‑150 (MQ=255)

                                          gATGGTGCCGGGGGCATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgccag                                                                                                  <  1:589923/150‑1 (MQ=255)

                                            tGGTGCCGGGTGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAgccaggc                                                                                                <  2:2917978/150‑1 (MQ=255)

                                                 ccGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCagaa                                                                                           <  2:2375744/150‑1 (MQ=255)

                                                  cGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCagaaa                                                                                          <  1:4853692/150‑1 (MQ=255)

                                                     gggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCagaaagaa                                                                                       <  2:4054815/150‑1 (MQ=255)

                                                          tGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCa                                                                                  <  2:2827339/150‑1 (MQ=255)

                                                           ggCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAg                                                                                 <  1:4248742/150‑1 (MQ=255)

                                                            gCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAgg                                                                                >  1:3022298/1‑150 (MQ=255)

                                                            gCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAgg                                                                                >  1:2590021/1‑150 (MQ=255)

                                                             ccGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGc                                                                               >  2:531884/1‑150 (MQ=255)

                                                             ccGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGc                                                                               <  1:1209105/150‑1 (MQ=255)

                                                             ccGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGc                                                                               <  1:2712389/150‑1 (MQ=255)

                                                               gATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGccc                                                                             >  1:737221/1‑150 (MQ=255)

                                                               gATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGccc                                                                             >  2:1334667/1‑150 (MQ=255)

                                                                    ggATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCccaccac                                                                        >  2:547782/1‑150 (MQ=255)

                                                                    ggATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCccaccac                                                                        >  2:3135282/1‑150 (MQ=255)

                                                                      aTCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCccaccacca                                                                      <  1:2249879/150‑1 (MQ=255)

                                                                         tGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagca                                                                   <  1:3923101/150‑1 (MQ=255)

                                                                          gCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcag                                                                  >  2:1303386/1‑150 (MQ=255)

                                                                           cTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                 <  2:4776069/150‑1 (MQ=255)

                                                                              cTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAgg                                                              <  1:913222/150‑1 (MQ=255)

                                                                              cTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAgg                                                              <  2:2662882/150‑1 (MQ=255)

                                                                               ttGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGc                                                             >  2:381252/1‑150 (MQ=255)

                                                                                   ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCaa                                                         >  1:4142652/1‑150 (MQ=255)

                                                                                   ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCaa                                                         >  1:3328786/1‑150 (MQ=255)

                                                                                   ccACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCaa                                                         >  2:1082042/1‑150 (MQ=255)

                                                                                    cACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAg                                                        <  1:2302785/150‑1 (MQ=255)

                                                                                     aCCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCCGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGa                                                       >  2:81743/1‑150 (MQ=255)

                                                                                     aCCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGTAGGCCCAAGa                                                       <  2:5066955/150‑1 (MQ=255)

                                                                                     aCCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGa                                                       >  1:4410101/1‑150 (MQ=255)

                                                                                       cTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCCAGAcc                                                     >  1:3824192/1‑150 (MQ=255)

                                                                                       cTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGAcc                                                     >  2:216787/1‑150 (MQ=255)

                                                                                        tGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCa                                                    <  2:372687/150‑1 (MQ=255)

                                                                                        tGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGACTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCa                                                    >  2:2535868/1‑150 (MQ=255)

                                                                                            ccAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTg                                                >  1:4210482/1‑150 (MQ=255)

                                                                                             cagcagcagGCGGTCACGCGGGCCGATGGCACGGCTGCCGACCATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCCCCAGCAGCAGGCCCAAGACCATCTgc                                               >  1:2319269/1‑150 (MQ=255)

                                                                                              agcagcagGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTgcg                                              >  1:3948605/1‑150 (MQ=255)

                                                                                               gcagcagGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCCTCTgcgc                                             >  1:4251919/1‑150 (MQ=255)

                                                                                               gcagcagGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTgcgc                                             >  1:4326708/1‑150 (MQ=255)

                                                                                                        ggTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGccc                                    <  2:867464/150‑1 (MQ=255)

                                                                                                             cgcgGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCaa                               <  1:282518/150‑1 (MQ=255)

                                                                                                             cgcgGGCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCaa                               <  1:282564/150‑1 (MQ=255)

                                                                                                                gggCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGAc                            <  2:2000360/150‑1 (MQ=255)

                                                                                                                gggCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGAc                            >  1:2722649/1‑150 (MQ=255)

                                                                                                                gggCCGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGAc                            >  1:135767/1‑150 (MQ=255)

                                                                                                                    cGATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGcc                        >  1:346035/1‑150 (MQ=255)

                                                                                                                     gATGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCa                       <  2:1527041/150‑1 (MQ=255)

                                                                                                                       tGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAgg                     <  1:5116564/150‑1 (MQ=255)

                                                                                                                       tGGCACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAgg                     <  2:3614564/150‑1 (MQ=255)

                                                                                                                          cACGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTg                  <  2:242926/150‑1 (MQ=255)

                                                                                                                           aCGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                 <  1:2242660/150‑1 (MQ=255)

                                                                                                                           aCGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                 <  2:1949732/150‑1 (MQ=255)

                                                                                                                           aCGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                 <  2:4395847/150‑1 (MQ=255)

                                                                                                                           aCGGCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                 <  2:2939572/150‑1 (MQ=255)

                                                                                                                             ggCTGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgc               <  1:2134653/150‑1 (MQ=255)

                                                                                                                                tGCCGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgccgc            <  2:1750270/150‑1 (MQ=255)

                                                                                                                                  ccGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGccgccg          >  2:10026/1‑150 (MQ=255)

                                                                                                                                  ccGACAATCTCGAT‑ACCTGCCAACCCTTCACTCCGGTGCTCTGAAG‑GCGCAGCAGCAGACAGGCCAGAAAGAAGATCAGGCCCACCACCAGCACCAGGCCCAAAAACATCTGCGCCAGCTGCCCGCCAAGACAGCCAGGTTGAGccgccg          >  1:823449/1‑150 (MQ=255)

                                                                                                                                  ccGACAATCTCGA‑GACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGccgccg          <  2:1497934/150‑1 (MQ=255)

                                                                                                                                   cGACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGc                                                                               >  2:757005/1‑80 (MQ=255)

                                                                                                                                    gACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCggg        >  2:910163/1‑150 (MQ=255)

                                                                                                                                    gACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCggg        >  2:3278016/1‑150 (MQ=255)

                                                                                                                                    gACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCggg        >  2:2969180/1‑150 (MQ=255)

                                                                                                                                    gACAATCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCggg        >  2:1815304/1‑150 (MQ=255)

                                                                                                                                       aaTCTCGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTg     >  2:3953316/1‑150 (MQ=255)

                                                                                                                                       aaTCTCGAT‑ACCTGCCCAACATTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCAAAGACAATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTg     >  2:482782/1‑150 (MQ=255)

                                                                                                                                         tctcGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGGCCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc   >  1:791607/1‑150 (MQ=255)

                                                                                                                                         tctcGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc   >  1:1088386/1‑150 (MQ=255)

                                                                                                                                         tctcGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc   >  2:2834573/1‑150 (MQ=255)

                                                                                                                                          ctcGAT‑ACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgct  >  2:5000391/1‑150 (MQ=255)

TGCCGGCACTTCCACGGGTTCGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCGCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCT  >  NC_002947/4948536‑4948824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`