Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,852,047 C→A A364D (GCC→GAC)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,852,0470CA99.5% 797.0 / ‑4.8 219A364D (GCC→GAC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (116/102);  total (116/103)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 224 total aligned reads displayed.

CCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGC  >  NC_002947/1851900‑1852193
                                                                                                                                                   |                                                                                                                                                  
ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGacc                                                                                                                                                  >  2:1572550/1‑150 (MQ=255)
  aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTCCCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCAc                                                                                                                                                >  1:3229620/1‑150 (MQ=255)
  aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCAc                                                                                                                                                >  2:4737148/1‑150 (MQ=255)
    ggAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTg                                                                                                                                              <  2:1536624/150‑1 (MQ=255)
    ggAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTg                                                                                                                                              <  1:4097508/150‑1 (MQ=255)
        ttCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGgcgc                                                                                                                                          >  2:119754/1‑150 (MQ=255)
        ttCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGgcgc                                                                                                                                          >  1:2354940/1‑150 (MQ=255)
           gacgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTAc                                                                                                                                       >  1:3451011/1‑150 (MQ=255)
            acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACa                                                                                                                                      <  2:1105358/150‑1 (MQ=255)
            acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACa                                                                                                                                      <  2:3045145/150‑1 (MQ=255)
            acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACa                                                                                                                                      >  1:3978293/1‑150 (MQ=255)
            acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACa                                                                                                                                      >  1:2719059/1‑150 (MQ=255)
             cgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACaa                                                                                                                                     <  2:3846362/150‑1 (MQ=255)
                   cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCAc                                                                                                                               <  1:2536366/150‑1 (MQ=255)
                    aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCAcc                                                                                                                              >  2:2479026/1‑150 (MQ=255)
                    aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTCCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCAcc                                                                                                                              >  2:3543052/1‑150 (MQ=255)
                       gCCAACCCCACGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGAc                                                                                                                           <  2:331397/150‑1 (MQ=255)
                       gCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGAc                                                                                                                           >  1:3575280/1‑150 (MQ=255)
                       gCCAACCCCAAGGAGAACGGCTACCGCGCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACGTGGCGTATGCGACCACTGGCGCTACAAGGGCACCgcc                                                                                                                           >  2:377592/1‑148 (MQ=255)
                            ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCaa                                                                                                                      <  2:4240069/150‑1 (MQ=255)
                             cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAg                                                                                                                     <  2:4810355/150‑1 (MQ=255)
                              ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGc                                                                                                                    >  2:1484724/1‑150 (MQ=255)
                                   gagaACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGc                                                                                                               <  2:4336092/150‑1 (MQ=255)
                                      aaCGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTcc                                                                                                            >  2:1058119/1‑150 (MQ=255)
                                         ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAc                                                                                                         >  2:840188/1‑150 (MQ=255)
                                           cTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGTAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCa                                                                                                       >  1:532527/1‑150 (MQ=255)
                                           cTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCa                                                                                                       >  2:4638118/1‑150 (MQ=255)
                                             aCCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACt                                                                                                     >  2:1443305/1‑150 (MQ=255)
                                               cGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTAc                                                                                                   >  2:2007298/1‑150 (MQ=255)
                                                    gTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaaga                                                                                              >  1:3765008/1‑150 (MQ=255)
                                                     ttGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                                                             >  1:3112819/1‑150 (MQ=255)
                                                     ttGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                                                             <  2:1689522/150‑1 (MQ=255)
                                                     ttGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                                                             <  2:3409884/150‑1 (MQ=255)
                                                     gtGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGATCTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                                                             <  1:1436125/149‑1 (MQ=255)
                                                      tGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaa                                                                                            <  2:1425768/150‑1 (MQ=255)
                                                       gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                                                           >  2:4600518/1‑150 (MQ=255)
                                                         acacTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGa                                                                                         <  1:752517/150‑1 (MQ=255)
                                                         acacTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGa                                                                                         <  1:3360159/150‑1 (MQ=255)
                                                             tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                     <  2:776744/150‑1 (MQ=255)
                                                             tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                     <  2:2015826/150‑1 (MQ=255)
                                                             tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                     <  1:99930/150‑1 (MQ=255)
                                                               cGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCt                                                                                   <  2:2259425/150‑1 (MQ=255)
                                                                ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                                                                  >  2:619070/1‑150 (MQ=255)
                                                                ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                                                                  <  2:929951/150‑1 (MQ=255)
                                                                   aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGtt                                                                               <  1:3549997/150‑1 (MQ=255)
                                                                   aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGtt                                                                               >  1:2561690/1‑150 (MQ=255)
                                                                        gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                          >  1:4041394/1‑150 (MQ=255)
                                                                        gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                          >  1:2007506/1‑150 (MQ=255)
                                                                         ccccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGCCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCa                                                                         >  2:2292653/1‑150 (MQ=255)
                                                                          cccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAg                                                                        <  2:3800347/150‑1 (MQ=255)
                                                                           ccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAgg                                                                       >  1:3892083/1‑150 (MQ=255)
                                                                           ccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAgg                                                                       >  1:3697496/1‑150 (MQ=255)
                                                                                ggCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTg                                                                  >  2:4029677/1‑150 (MQ=255)
                                                                                  cAAGGTGCTGGAGGTGCAGATCCGTTCCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGa                                                                <  2:321876/150‑1 (MQ=255)
                                                                                   aaGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAg                                                               >  1:835436/1‑150 (MQ=255)
                                                                                      gTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTgg                                                            >  1:3077823/1‑150 (MQ=255)
                                                                                       tGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGc                                                           <  2:4728196/150‑1 (MQ=255)
                                                                                        gCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa                                                          >  1:164875/1‑150 (MQ=255)
                                                                                        gCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa                                                          <  2:5024724/150‑1 (MQ=255)
                                                                                          tGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACg                                                        >  1:4812641/1‑150 (MQ=255)
                                                                                           ggAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAAGTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAATAGATTTCCTGGTTGCGTCAGGTGTTGGAGTGGCaggt                                                       >  2:3588510/1‑147 (MQ=255)
                                                                                            gAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCAGAACATGGCGTATGCGACCACTGGCGCTAAAAGGGCACCGACGTCAAGCCCAGCTCCAAACACTACGAAGAAAAGATTTCCTGTTTGCGTCAGGTGCTGGAGTGGAAAGaa                                                      >  1:2441196/1‑150 (MQ=255)
                                                                                                 gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt                                                 >  1:2366825/1‑150 (MQ=255)
                                                                                                   aGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg                                               <  2:1784209/150‑1 (MQ=255)
                                                                                                   aGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg                                               <  1:4628481/150‑1 (MQ=255)
                                                                                                    gATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTggg                                              >  2:3821839/1‑150 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                     >  2:5031041/1‑110 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                     <  1:5031041/110‑1 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc                                             >  1:1915710/1‑150 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc                                             <  1:1465281/150‑1 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc                                             <  1:1040666/150‑1 (MQ=255)
                                                                                                     aTCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc                                             <  1:4829198/150‑1 (MQ=255)
                                                                                                       ccGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGa                                           <  1:2860532/150‑1 (MQ=255)
                                                                                                         ntACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACa                                         >  1:632248/2‑150 (MQ=255)
                                                                                                           aCCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATc                                       <  1:4246094/150‑1 (MQ=255)
                                                                                                             ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg                                     >  1:2611501/1‑150 (MQ=255)
                                                                                                             ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg                                     >  2:1892679/1‑150 (MQ=255)
                                                                                                             ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg                                     >  1:4537772/1‑150 (MQ=255)
                                                                                                              cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt                                    <  1:2982202/150‑1 (MQ=255)
                                                                                                              cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt                                    <  1:1346005/150‑1 (MQ=255)
                                                                                                              cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt                                    >  2:5009096/1‑150 (MQ=255)
                                                                                                               aCGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtg                                   >  2:361807/1‑150 (MQ=255)
                                                                                                                cGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATATCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtgg                                  >  2:4524262/1‑150 (MQ=255)
                                                                                                                 ggCATGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggc                                 >  1:4980040/1‑150 (MQ=255)
                                                                                                                    aTGCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctg                              >  2:3973071/1‑150 (MQ=255)
                                                                                                                      gCACGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctggc                            >  2:4794623/1‑150 (MQ=255)
                                                                                                                        aCGAAGAGGCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCg                          >  1:3129042/1‑150 (MQ=255)
                                                                                                                               ggCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGtt                   <  2:4882613/150‑1 (MQ=255)
                                                                                                                                gCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTg                  >  1:1529108/1‑150 (MQ=255)
                                                                                                                                gCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTg                  <  2:164875/150‑1 (MQ=255)
                                                                                                                                gCCGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTg                  >  2:1793072/1‑150 (MQ=255)
                                                                                                                                 ccGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTCCGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGc                 <  2:795795/150‑1 (MQ=255)
                                                                                                                                 ccGAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGc                 <  1:4737148/150‑1 (MQ=255)
                                                                                                                                   gAACTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg               <  2:3358140/150‑1 (MQ=255)
                                                                                                                                      cTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc            <  2:3451011/150‑1 (MQ=255)
                                                                                                                                      cTTGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc            <  2:5149217/150‑1 (MQ=255)
                                                                                                                                       ttGGCGTATGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCg           >  1:4336803/1‑150 (MQ=255)
                                                                                                                                              aTGCGACCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGa    <  1:3324281/150‑1 (MQ=255)
                                                                                                                                                gcgaCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGACGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGc  >  1:1800937/1‑150 (MQ=255)
                                                                                                                                                gcgaCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGc  <  2:1845459/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGC  >  NC_002947/1851900‑1852193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑